目的 探讨围手术期应用鱼油对大鼠“术后疲劳”的影响.方法 60只SD大鼠,经术前1周行为学训练,以其结果为依据筛选出表现较差的12只为血清空白对照组,余48只按随机数字表法随机均分为模型组和鱼油干预组.鱼油干预组于术前3d至术后7d腹腔注射鱼油[2 ml/(kg·d)],模型组同时段注射生理盐水[2 ml/ (kg·d)],术后1、3、5、7d,Morris水迷宫和悬尾实验行为学评估,检测外周血白介素(IL)-1β、IL-6、肿瘤坏死因子-α(TNF-α)和超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-PX)活力.结果 血清指标:鱼油干预组术后1、3 d IL-6[(66.22±8.80)、(56.03±1.19) pg/ml]明显低于模型组[(83.30±10.69)、(82.72±24.27)pg/ml](PP=0.034,P=0.038)；术后1、3、5、7 dTNF-α[ (104.36±5.02)、(84.49±7.81)、(64.47±2.89)、(39.29±2.52) pg/ml]明显低于模型组[(120.01±14.99)、(119.68±8.84)、(75.29±2.58)、(41.96±1.65) pg/ml] (P=0.014,P=0.003,P=0.000,P=0.004)；术后1、3、5、7 d IL-1β[(155.11±9.08)、(79.39±5.86)、(57.26±16.07)、(35.42±1.53) pg/ml]明显低于模型组[(204.87±30.61)、(198.82±54.83)、(152.12±29.06)、(64.35±2.70) pg/ml] (P=0.024,P=0.002,P=0.000,P=0.000)；术后5dSOD (1.08±0.08) μmol/L明显高于模型组(0.71±0.06) μmol/L(P=0.000)；术后5、7 d GSH-PX[(31.21±1.30)、(30.78±1.83) μmol/L]明显高于模型组[(25.03±1.74)、(27.57±3.57) μmol/L] (P=0.000,P=0.036).行为学:悬尾实验鱼油干预组术后1、3d挣扎阈上面积[(6620±1390)、(7011±1472) mv·8]较模型组[(4739±1040)、(4344±1130) mv·s]明显增加(P=0.048,P=0.043)；累积不动时间[ (118.42±10.05)、 (101.02±8.68)s]和单次最长静止时间[(55.39±7.70),(56.60±5.88)s]较模型组[(135.08±12.44)、(131.02±9.24)s；(65.73±3.78)、(64.93±3.25)s]明显减少(P =0.042,P=0.012,P=0.043,P=0.042).Morris水迷宫鱼油干预组术后3、5d逃避潜伏期[ (48.263±1.815)、(44.955±2.567)s]较模型组[(51.543±1.990)、(49.956±2.888)s]明显减少(P=0.035,P=0.035)；术后1、3、5、7d穿越平台次数[(1.04±0.25)、(1.95±0.49)、(2.42±0.41)、(3.21±0.53)次]较模型组[(0.58±0.26)、(1.20±0.33)、(1.50±0.39)、(2.17±0.68)次]明显增加(P =0.002,P=0.003,P=0.018,P=0.035).结论 围手术期鱼油干预能有效改善大鼠术后的炎症反应,可能增强抗氧化防御能力,改善“术后疲劳”.
目的 探討圍手術期應用魚油對大鼠“術後疲勞”的影響.方法 60隻SD大鼠,經術前1週行為學訓練,以其結果為依據篩選齣錶現較差的12隻為血清空白對照組,餘48隻按隨機數字錶法隨機均分為模型組和魚油榦預組.魚油榦預組于術前3d至術後7d腹腔註射魚油[2 ml/(kg·d)],模型組同時段註射生理鹽水[2 ml/ (kg·d)],術後1、3、5、7d,Morris水迷宮和懸尾實驗行為學評估,檢測外週血白介素(IL)-1β、IL-6、腫瘤壞死因子-α(TNF-α)和超氧化物歧化酶(SOD)及穀胱甘肽過氧化物酶(GSH-PX)活力.結果 血清指標:魚油榦預組術後1、3 d IL-6[(66.22±8.80)、(56.03±1.19) pg/ml]明顯低于模型組[(83.30±10.69)、(82.72±24.27)pg/ml](PP=0.034,P=0.038)；術後1、3、5、7 dTNF-α[ (104.36±5.02)、(84.49±7.81)、(64.47±2.89)、(39.29±2.52) pg/ml]明顯低于模型組[(120.01±14.99)、(119.68±8.84)、(75.29±2.58)、(41.96±1.65) pg/ml] (P=0.014,P=0.003,P=0.000,P=0.004)；術後1、3、5、7 d IL-1β[(155.11±9.08)、(79.39±5.86)、(57.26±16.07)、(35.42±1.53) pg/ml]明顯低于模型組[(204.87±30.61)、(198.82±54.83)、(152.12±29.06)、(64.35±2.70) pg/ml] (P=0.024,P=0.002,P=0.000,P=0.000)；術後5dSOD (1.08±0.08) μmol/L明顯高于模型組(0.71±0.06) μmol/L(P=0.000)；術後5、7 d GSH-PX[(31.21±1.30)、(30.78±1.83) μmol/L]明顯高于模型組[(25.03±1.74)、(27.57±3.57) μmol/L] (P=0.000,P=0.036).行為學:懸尾實驗魚油榦預組術後1、3d掙扎閾上麵積[(6620±1390)、(7011±1472) mv·8]較模型組[(4739±1040)、(4344±1130) mv·s]明顯增加(P=0.048,P=0.043)；纍積不動時間[ (118.42±10.05)、 (101.02±8.68)s]和單次最長靜止時間[(55.39±7.70),(56.60±5.88)s]較模型組[(135.08±12.44)、(131.02±9.24)s；(65.73±3.78)、(64.93±3.25)s]明顯減少(P =0.042,P=0.012,P=0.043,P=0.042).Morris水迷宮魚油榦預組術後3、5d逃避潛伏期[ (48.263±1.815)、(44.955±2.567)s]較模型組[(51.543±1.990)、(49.956±2.888)s]明顯減少(P=0.035,P=0.035)；術後1、3、5、7d穿越平檯次數[(1.04±0.25)、(1.95±0.49)、(2.42±0.41)、(3.21±0.53)次]較模型組[(0.58±0.26)、(1.20±0.33)、(1.50±0.39)、(2.17±0.68)次]明顯增加(P =0.002,P=0.003,P=0.018,P=0.035).結論 圍手術期魚油榦預能有效改善大鼠術後的炎癥反應,可能增彊抗氧化防禦能力,改善“術後疲勞”.
목적 탐토위수술기응용어유대대서“술후피로”적영향.방법 60지SD대서,경술전1주행위학훈련,이기결과위의거사선출표현교차적12지위혈청공백대조조,여48지안수궤수자표법수궤균분위모형조화어유간예조.어유간예조우술전3d지술후7d복강주사어유[2 ml/(kg·d)],모형조동시단주사생리염수[2 ml/ (kg·d)],술후1、3、5、7d,Morris수미궁화현미실험행위학평고,검측외주혈백개소(IL)-1β、IL-6、종류배사인자-α(TNF-α)화초양화물기화매(SOD)급곡광감태과양화물매(GSH-PX)활력.결과 혈청지표:어유간예조술후1、3 d IL-6[(66.22±8.80)、(56.03±1.19) pg/ml]명현저우모형조[(83.30±10.69)、(82.72±24.27)pg/ml](PP=0.034,P=0.038)；술후1、3、5、7 dTNF-α[ (104.36±5.02)、(84.49±7.81)、(64.47±2.89)、(39.29±2.52) pg/ml]명현저우모형조[(120.01±14.99)、(119.68±8.84)、(75.29±2.58)、(41.96±1.65) pg/ml] (P=0.014,P=0.003,P=0.000,P=0.004)；술후1、3、5、7 d IL-1β[(155.11±9.08)、(79.39±5.86)、(57.26±16.07)、(35.42±1.53) pg/ml]명현저우모형조[(204.87±30.61)、(198.82±54.83)、(152.12±29.06)、(64.35±2.70) pg/ml] (P=0.024,P=0.002,P=0.000,P=0.000)；술후5dSOD (1.08±0.08) μmol/L명현고우모형조(0.71±0.06) μmol/L(P=0.000)；술후5、7 d GSH-PX[(31.21±1.30)、(30.78±1.83) μmol/L]명현고우모형조[(25.03±1.74)、(27.57±3.57) μmol/L] (P=0.000,P=0.036).행위학:현미실험어유간예조술후1、3d쟁찰역상면적[(6620±1390)、(7011±1472) mv·8]교모형조[(4739±1040)、(4344±1130) mv·s]명현증가(P=0.048,P=0.043)；루적불동시간[ (118.42±10.05)、 (101.02±8.68)s]화단차최장정지시간[(55.39±7.70),(56.60±5.88)s]교모형조[(135.08±12.44)、(131.02±9.24)s；(65.73±3.78)、(64.93±3.25)s]명현감소(P =0.042,P=0.012,P=0.043,P=0.042).Morris수미궁어유간예조술후3、5d도피잠복기[ (48.263±1.815)、(44.955±2.567)s]교모형조[(51.543±1.990)、(49.956±2.888)s]명현감소(P=0.035,P=0.035)；술후1、3、5、7d천월평태차수[(1.04±0.25)、(1.95±0.49)、(2.42±0.41)、(3.21±0.53)차]교모형조[(0.58±0.26)、(1.20±0.33)、(1.50±0.39)、(2.17±0.68)차]명현증가(P =0.002,P=0.003,P=0.018,P=0.035).결론 위수술기어유간예능유효개선대서술후적염증반응,가능증강항양화방어능력,개선“술후피로”.
Objective To investigate the effect of perioperative use of fish oil on "post-operative fatigue"(POF) of rat.Methods After one week's preoperative behavior training,12 rats presented poor behavior were excluded from 60 healthy adult male SD rats as the normal controls of serum parameters.The remaining 48 rats were randomly divided into model group and fish oil treatment group by random number table.The fish oil treatment group received 10 days' (3 days before surgery and 7 days after surgery) intraperitoneal injection of fish oil [2 ml/( kg · d) ],and the model group with saline.On the 1st,3rd,5th,and 7th post-operative day,rats were assessed by Morris water-maze and tail suspension test.Serum levels of interleukin ( IL)-1β,IL-6,tumor necrosis factor-α (TNF-α),superoxide dismutase (SOD),and glutathione peroxidase (GSH-PX) were measured.Results Serum parameters:on the 1st and 3rd post-operative day,the IL-6 level in the fish oil treatment group [ (66.22 ±8.80),(56.03 ± 1.19) pg/ml] was significantly lower than in model group [ (83.30 ± 10.69),(82.72 ± 24.27) pg/ml ] (P =0.034,P =0.038 ) ; on the 1 st,3rd,5th,and 7th post-operative day,the TNF-α level in the fish oil treatment group [ ( 104.36 ±5.02),(84.49 ±7.81 ),(64.47 ±2.89),(39.29 ±2.52)pg/ml ] was significantly lower than in model group [ ( 120.01 ± 14.99 ),( 119.68 ± 8.84),(75.29 ± 2.58 ),(41.96±1.65) pg/ml] (P=0.014,P=0.003,P=0.000,P=0.004); onthe1st,3rd,5th,and 7th postoperative day,the IL-1β level [(155.11 ±9.08),(79.39±5.86),(57.26±16.07),(35.42±1.53) pg/ml]was significantly lower than model group [ (204.87±30.61), (198.82±54.83), (152.12±29.06),(64.35 ± 2.70) pg/ml ] ( P =0.024,P =0.002,P =0.000,P =0.000) ; on the 5th postoperative day,SOD ( 1.08±0.08) μmol/L was significantly higher than model group (0.71±0.06) μmoL/L (P=0.000) ; on the 5th and 7th postoperative day,GSH-PX [ (31.21 ± 1.30), (30.78 ± 1.83) μmol/L] was significantly higher than model group [ (25.03 ±1.74),(27.57±3.57) μ mol/L] (P=0.000,P=0.036).Behavior:in tail suspension test,on the 1st and 3rd postoperative day,value of struggle in fish oil treatment group [ (6620 ± 1390),(7011 ± 1472) mv · s] was significantly higher than in model group [ (4739 ± 1040),(4344 ± 1130) mv · s](P=0.048,P=0.043); cumulative fixed time [ (118.42±10.05), (101.02±8.68) s] and single rest time [ (55.39±7.70),(56.60±5.88) s] was lower thanin modelgroup [ (135.08+12.44),(131.02±9.24) s; (65.73±3.78),(64.93±3.25) s] (P=0.042,P=0.012,P=0.043,and P=0.042).In Morris water-maze,on the 3rd and 5th postoperative day,escape latent period of fish oil treatment group [ (48.263 ±1.815),(44.955±2.567) s] was lower than model group [ (51.543±1.990),(49.956±2.888) s] (P=0.035,P=0.035) ; on the 1st,3rd,5th,and 7th postoperative day,the cross platform number (1.04±0.25,1.95±0.49,2.42 ±0.41,3.21 ±0.53) was significantly higher than in model group (0.58 ±0.26,1.20±0.33,1.50±0.39,2.17±0.68) (P=0.002,P=0.003,P=0.018,P=0.035).Conclusion Perioperative use of fish oil can reduce postoperative inflammatory response,enhance antioxidant defense capability,and mitigate post-operative fatigue.
