中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2012年
2期
128-131
,共4页
陈磊%匡瑞霞%房晶%赵文君%李慧超
陳磊%劻瑞霞%房晶%趙文君%李慧超
진뢰%광서하%방정%조문군%리혜초
端粒酶%肉芽组织%瘢痕疙瘩%成熟性瘢痕%增生性瘢痕
耑粒酶%肉芽組織%瘢痕疙瘩%成熟性瘢痕%增生性瘢痕
단립매%육아조직%반흔흘탑%성숙성반흔%증생성반흔
Telomerase%Granulation tissue%Keloid%Mature scar%Hypertrophic scar
目的 通过研究瘢痕发生、发展过程中成纤维细胞中端粒酶的表达,探讨端粒酶与瘢痕发生、发展的关系.方法 采用免疫组织化学染色法(PV-6000二步法).实验分为5组:肉芽组织组18例、瘢痕疙瘩组17例、增生性瘢痕组16例、成熟性瘢痕组28例、正常皮肤组32例,对标本成纤维细胞端粒酶活性进行检测,用SPSS16.0进行统计学分析.结果 肉芽组织组成纤维细胞端粒酶阳性表达率为94.4％,瘢痕疙瘩组阳性表达率为58.8％,增生性瘢痕组阳性率为18.8％,成熟性瘢痕组阳性率为0,正常皮肤组阳性率为0,各组间两两比较,除成熟性瘢痕组和正常皮肤组外,其余各组差别均有统计学意义(P＜0.05).结论 瘢痕的形成是一个多因素参与综合作用的结果,而端粒酶的激活是其中一个重要的因素.通过检测瘢痕发生、发展过程中的端粒酶活性,有可能判断瘢痕的预后;通过调控瘢痕发生、发展过程中端粒酶的活性,有可能成为瘢痕治疗的新途径.
目的 通過研究瘢痕髮生、髮展過程中成纖維細胞中耑粒酶的錶達,探討耑粒酶與瘢痕髮生、髮展的關繫.方法 採用免疫組織化學染色法(PV-6000二步法).實驗分為5組:肉芽組織組18例、瘢痕疙瘩組17例、增生性瘢痕組16例、成熟性瘢痕組28例、正常皮膚組32例,對標本成纖維細胞耑粒酶活性進行檢測,用SPSS16.0進行統計學分析.結果 肉芽組織組成纖維細胞耑粒酶暘性錶達率為94.4％,瘢痕疙瘩組暘性錶達率為58.8％,增生性瘢痕組暘性率為18.8％,成熟性瘢痕組暘性率為0,正常皮膚組暘性率為0,各組間兩兩比較,除成熟性瘢痕組和正常皮膚組外,其餘各組差彆均有統計學意義(P＜0.05).結論 瘢痕的形成是一箇多因素參與綜閤作用的結果,而耑粒酶的激活是其中一箇重要的因素.通過檢測瘢痕髮生、髮展過程中的耑粒酶活性,有可能判斷瘢痕的預後;通過調控瘢痕髮生、髮展過程中耑粒酶的活性,有可能成為瘢痕治療的新途徑.
목적 통과연구반흔발생、발전과정중성섬유세포중단립매적표체,탐토단립매여반흔발생、발전적관계.방법 채용면역조직화학염색법(PV-6000이보법).실험분위5조:육아조직조18례、반흔흘탑조17례、증생성반흔조16례、성숙성반흔조28례、정상피부조32례,대표본성섬유세포단립매활성진행검측,용SPSS16.0진행통계학분석.결과 육아조직조성섬유세포단립매양성표체솔위94.4％,반흔흘탑조양성표체솔위58.8％,증생성반흔조양성솔위18.8％,성숙성반흔조양성솔위0,정상피부조양성솔위0,각조간량량비교,제성숙성반흔조화정상피부조외,기여각조차별균유통계학의의(P＜0.05).결론 반흔적형성시일개다인소삼여종합작용적결과,이단립매적격활시기중일개중요적인소.통과검측반흔발생、발전과정중적단립매활성,유가능판단반흔적예후;통과조공반흔발생、발전과정중단립매적활성,유가능성위반흔치료적신도경.
Objective To study the expression of telomerase of fibroblasts in the formation and development of scar,and to investgate the relationship between the telomerase activity and the formation and development of scar.Methods Expression of telomerase was detected by immunohistochemical techniques in 18 specimens from granulation tissue,17 keloid,16 specimens from hypertrophic scar,28 specimens from mature scar,32 specimens from normal skin,and SPSS16.0 statistics software was used to analyze the relationship between telomerase and scar.Results It was shown that the positive expression rate of telomerase in the granulation tissue group was 94.4 ％,that in the keloid group was 58.8 ％,that in the hypertrophic scar group was 18.8 ％, and that in the mature scar and in the normal skin was zero. comparisons between groups,in addition to the normal scar group and normal skin group,other groups were statistically significant differences.Conclnsions Scar formation is a process with multi-factor participation,while telomerase activation is an important factor. Detecting telomerase activity in the development process of scar formation may determine the prognosis.Through the control of telomerase activity in the pathogenesis of scar may become a new approach of scar treatment.
