中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
3期
465-467
,共3页
吴建军%邢德国%王亮%田克立%刘中浩%宫明智
吳建軍%邢德國%王亮%田剋立%劉中浩%宮明智
오건군%형덕국%왕량%전극립%류중호%궁명지
骨形态发生蛋白-2%胰岛素样生长因子-1%骨髓基质干细胞%高糖%细胞增殖
骨形態髮生蛋白-2%胰島素樣生長因子-1%骨髓基質榦細胞%高糖%細胞增殖
골형태발생단백-2%이도소양생장인자-1%골수기질간세포%고당%세포증식
BMP-2%IGF-1%BMSC%High glucose%Cell proliferation
目的 观察高糖环境下骨形态发生蛋白-2(BMP-2)和胰岛素样生长因子-1(IGF-1)基因转染大鼠骨髓基质干细胞(BMSC)后BMSC的增殖.方法 用Ad-BMP-2和Ad-IGF-1转染大鼠BMSC,Wester blot检测蛋白表达.噻唑蓝(MTT)比色法及流式细胞术检测细胞增殖.结果 Western blot检测到转染组细胞中有目的 蛋白表达.MTT结果显示第5天细胞增殖能力达到高峰,5 d光吸收值:A～E组分别为0.324±0.027、0.319±0.017、0.622±0.028、0.626±0.020、0.778±0.031.流式细胞术结果显示A～E组细胞处于增殖期的比重分别为23.92±3.07、23.51±2.11、34.37±6.85、35.04±1.45、42.56±1.15.结论 高糖环境下BMP-2和IGF-1基因转染均能促进BMSC增殖,联合转染对BMSC增殖有协同效应.
目的 觀察高糖環境下骨形態髮生蛋白-2(BMP-2)和胰島素樣生長因子-1(IGF-1)基因轉染大鼠骨髓基質榦細胞(BMSC)後BMSC的增殖.方法 用Ad-BMP-2和Ad-IGF-1轉染大鼠BMSC,Wester blot檢測蛋白錶達.噻唑藍(MTT)比色法及流式細胞術檢測細胞增殖.結果 Western blot檢測到轉染組細胞中有目的 蛋白錶達.MTT結果顯示第5天細胞增殖能力達到高峰,5 d光吸收值:A～E組分彆為0.324±0.027、0.319±0.017、0.622±0.028、0.626±0.020、0.778±0.031.流式細胞術結果顯示A～E組細胞處于增殖期的比重分彆為23.92±3.07、23.51±2.11、34.37±6.85、35.04±1.45、42.56±1.15.結論 高糖環境下BMP-2和IGF-1基因轉染均能促進BMSC增殖,聯閤轉染對BMSC增殖有協同效應.
목적 관찰고당배경하골형태발생단백-2(BMP-2)화이도소양생장인자-1(IGF-1)기인전염대서골수기질간세포(BMSC)후BMSC적증식.방법 용Ad-BMP-2화Ad-IGF-1전염대서BMSC,Wester blot검측단백표체.새서람(MTT)비색법급류식세포술검측세포증식.결과 Western blot검측도전염조세포중유목적 단백표체.MTT결과현시제5천세포증식능력체도고봉,5 d광흡수치:A～E조분별위0.324±0.027、0.319±0.017、0.622±0.028、0.626±0.020、0.778±0.031.류식세포술결과현시A～E조세포처우증식기적비중분별위23.92±3.07、23.51±2.11、34.37±6.85、35.04±1.45、42.56±1.15.결론 고당배경하BMP-2화IGF-1기인전염균능촉진BMSC증식,연합전염대BMSC증식유협동효응.
Objective To observe the proliferation of bone marrow stromal cell (BMSC) transfected by bone morphogenetic protein-2 (BMP-2) and insulin-like growth factor-1 ( IGF-1 ) gene under high glucose condition.Methods Ad-BMP-2 and Ad-IGF-1 transfected rat BMSC,protein expression of BMSC were detected by Western blotting analysis.Methyl thiazol tetrazolium (MTT) assay and flow cytometry to observe the proliferation potential of BMSC.Results In the Western blotting analysis,positive signal lane of protein was observed in transfected group.MTT assay show that proliferation reached the peak in all groups on the fifth day,and the absorbency values of A to E group were 0.324 ± 0.027,0.319 ± 0.017,0.622 ±0.028,0.626 ± 0.020,0.778 ± 0.031.Flow cytometry show that the proliferative percentage from A to E group were 23.92 ±3.07,23.51 ±2.11,34.37 ±6.85,35.04 ± 1.45,42.56 ± 1.15.Conclusion BMP-2 or IGF-1 can promote the proliferation of BMSC under high glucose condition,but the combined has the synergy effect.
