CAJ | 학술논문

目的观察大鼠在应激原去除后颞下颌关节髁突、关节盘超微结构的变化.方法 12周雄性Wistar大鼠32只,按完全随机设计分为实验组和对照组,各组16只,各组又随机分为9周组和12周组,各为8只大鼠.实验组前6周建立慢性不可预知性应激模型(chronic unpredictable stress,CUS),然后去除各种刺激因素,改为正常饲养;对照组始终正常饮食,不加任何刺激.9周、12周时间点分别处死9周组和12周组大鼠,完整分离大鼠左侧髁突和关节盘,制作电镜标本,扫描电镜下观察大鼠髁突及关节盘下腔面超微结构的变化.结果实验组大鼠髁突及关节盘下腔面扫描电镜下出现不同程度的恢复情况,表现为胶原纤维排列变得有规律,深层暴露减少,表面凝胶样物质增多等,对照组大鼠无明显变化.结论应激造成的大鼠颞下颌关节局部的病理损伤变化在一定时间内是可以恢复的,即该病理过程是可逆的.
목적 관찰대서재응격원거제후섭하합관절과돌、관절반초미결구적변화.방법 12주웅성Wistar대서32지,안완전수궤설계분위실험조화대조조,각조16지,각조우수궤분위9주조화12주조,각위8지대서.실험조전6주건립만성불가예지성응격모형(chronic unpredictable stress,CUS),연후거제각충자격인소,개위정상사양;대조조시종정상음식,불가임하자격.9주、12주시간점분별처사9주조화12주조대서,완정분리대서좌측과돌화관절반,제작전경표본,소묘전경하관찰대서과돌급관절반하강면초미결구적변화.결과 실험조대서과돌급관절반하강면소묘전경하출현불동정도적회복정황,표현위효원섬유배렬변득유규률,심층폭로감소,표면응효양물질증다등,대조조대서무명현변화.결론 응격조성적대서섭하합관절국부적병리손상변화재일정시간내시가이회복적,즉해병리과정시가역적.
Objective To examine the changes of the ultragtructures of temporomandibular joint after removal of the emotional stress factors in rats.Methods Thirty-two 12-week-old male Wistar rats were divided into two groups randomly,experimental group and control group.Each group was divided into two subgroups according to execution time,9-week subgroup and 12-week subgroup with eight rats in each subgroup.Chronic unpredictable stress animal model were firstly established in experimental group in the first 6 weeks,then all the stimulation factors removed and breed normally.After 9 weeks,rats in 9-week subgroup were killed.After 12 weeks,rats in 12-week subgroup were killed.All condyles and articular discs were dissected and observed by scanning electron microscope. Results There wag some recovery in condyles and articular discs in experimental group under scanning electron microscope.The gelatum on the surface of condyles increased,collagen fibrils became regular and deep layer collagen fibrils less exposed.There were no such obvious changes on the surface of condyles and articular discs in control group.Conclusions The ultrastructure injures of temporomandibular joint in rats induced by emotional stress could be reversed if the stress factors were removed.