中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2012年
1期
16-19
,共4页
邹俊%姜为民%毛海青%耿德春%朱雪松%干旻峰%许运%杨惠林
鄒俊%薑為民%毛海青%耿德春%硃雪鬆%榦旻峰%許運%楊惠林
추준%강위민%모해청%경덕춘%주설송%간민봉%허운%양혜림
慢病毒%骨髓间充质干细胞%脊柱融合%骨形态发生蛋白
慢病毒%骨髓間充質榦細胞%脊柱融閤%骨形態髮生蛋白
만병독%골수간충질간세포%척주융합%골형태발생단백
Lentivirus%Bone marrow mesenchymal stem cell%Spine fusion%Bone morphogenetic protein
目的 评价慢病毒转染的骨髓间充质干细胞诱导大鼠脊柱融合的能力.方法 构建携带骨形态发生蛋白2(BMP-2)的慢病毒并将其转染到骨髓间充质干细胞中,观察细胞的碱性磷酸酶染色和活性.并将转染的细胞移植到大鼠脊柱融合模型中,8周后处死大鼠,对其脊柱标本进行手触力学测试,Micro-CT扫描和组织学切片观察.结果 慢病毒转染细胞的碱性磷酸酶染色明显好于未转染细胞(3.2±0.4比1.1± 0.2,P＜0.05).转染的细胞移植到大鼠体内8周后,X线、Micro-CT、手触力学测试和组织学切片均显示转染的大鼠脊柱标本全部达到骨性融合.而未转染的大鼠脊柱标本均未融合.结论 慢病毒转染的骨髓间充质干细胞能够诱导脊柱融合,可作为基因载体工具用于脊柱融合.
目的 評價慢病毒轉染的骨髓間充質榦細胞誘導大鼠脊柱融閤的能力.方法 構建攜帶骨形態髮生蛋白2(BMP-2)的慢病毒併將其轉染到骨髓間充質榦細胞中,觀察細胞的堿性燐痠酶染色和活性.併將轉染的細胞移植到大鼠脊柱融閤模型中,8週後處死大鼠,對其脊柱標本進行手觸力學測試,Micro-CT掃描和組織學切片觀察.結果 慢病毒轉染細胞的堿性燐痠酶染色明顯好于未轉染細胞(3.2±0.4比1.1± 0.2,P＜0.05).轉染的細胞移植到大鼠體內8週後,X線、Micro-CT、手觸力學測試和組織學切片均顯示轉染的大鼠脊柱標本全部達到骨性融閤.而未轉染的大鼠脊柱標本均未融閤.結論 慢病毒轉染的骨髓間充質榦細胞能夠誘導脊柱融閤,可作為基因載體工具用于脊柱融閤.
목적 평개만병독전염적골수간충질간세포유도대서척주융합적능력.방법 구건휴대골형태발생단백2(BMP-2)적만병독병장기전염도골수간충질간세포중,관찰세포적감성린산매염색화활성.병장전염적세포이식도대서척주융합모형중,8주후처사대서,대기척주표본진행수촉역학측시,Micro-CT소묘화조직학절편관찰.결과 만병독전염세포적감성린산매염색명현호우미전염세포(3.2±0.4비1.1± 0.2,P＜0.05).전염적세포이식도대서체내8주후,X선、Micro-CT、수촉역학측시화조직학절편균현시전염적대서척주표본전부체도골성융합.이미전염적대서척주표본균미융합.결론 만병독전염적골수간충질간세포능구유도척주융합,가작위기인재체공구용우척주융합.
Objective To assess the spinal fusion in rats induced by bone marrow mesenchymal stem cells (BMSC) transfected with lentivirus.Methods Alkaline phosphatase (ALP) staining and ALP activity were determined in BMSC transfected with lentivirus encoding BMP-2 that were implanted to rat model of spinal fusion.At week 8,all rats were sacrificed.Thereafter,measurements of manual mechanics,Micro-CT scanning and histological analysis were performed.Results ALP staining appeared more satisfactory in BMSC transfected with lentivirus as compared with untransfected cells (3.2±0.4 vs 1.1 ± 0.2,P＜0.05).At week 8,spine bony fusion was achieved in all the transfected rats,as indicated by radiographic evaluation,Micro-CT and manual mechanics.In contrast,spinal fusion was absent in untransfected rats.Conclusion Lentivirus-transfected BMSC may achieve well-induced spinal fusion,and lentivirus vectors may offer the application of spinal fusion.
