CAJ | 학술논문

目的研究几种食源性吸虫间的系统发生关系.方法 PCR扩增华支睾吸虫和东方次睾吸虫18S rDNA片段并测序,从GenBank检索其他食源性吸虫和日本血吸虫的18S rDNA序列,利用CLUSTAL X软件比对后,用MEGA 5.0软件计算食源性吸虫间的遗传距离,并用邻接法、最大似然法和最大简约法构建系统发生树.结果共获得12种食源性吸虫序列,12种吸虫间遗传距离从0.001到0.083,系统发生树显示后睾科与异形科先聚在一起,然后与并殖科和双腔科聚成一支,片形科单独聚成一支.结论后睾科与异形科有较近的亲缘关系.
목적 연구궤충식원성흡충간적계통발생관계.방법 PCR확증화지고흡충화동방차고흡충18S rDNA편단병측서,종GenBank검색기타식원성흡충화일본혈흡충적18S rDNA서렬,이용CLUSTAL X연건비대후,용MEGA 5.0연건계산식원성흡충간적유전거리,병용린접법、최대사연법화최대간약법구건계통발생수.결과 공획득12충식원성흡충서렬,12충흡충간유전거리종0.001도0.083,계통발생수현시후고과여이형과선취재일기,연후여병식과화쌍강과취성일지,편형과단독취성일지.결론 후고과여이형과유교근적친연관계.
Objective To discuss the phylogeny of several food-borne trematodes.Methods The 18S rDNA partial sequences of Clonorchis sinensis and Metorchis orientalis were specifically amplified by PCR and then sequenced,the corresponding sequences of other food-borne trematodes were obtained from GenBank.After aligned by CLUSTAL X program all sequences were used to calculate the genetic distance and construct the phylogenic trees using neighbor-joining method,maximum parsimony method and maximum likelihood method in MEGA5.0 program.Results The 18S rDNA panial sequences of 12 species of food-bome trematode were obtained.The genetic distances among 12 trematodes were from 0.001 to 0.083.phylogenic trees showed that Opisthorchiidae and Hererophyidae polymerize first,then form a clader with Paragonimidae and Dicrocoeliidae,Fasciolidae form another clader.Conclusion Opisthorchiidae is closer to Hererophyidae in evolution.