中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2011年
4期
299-302
,共4页
赵娜%睢罡%李南春%武昕%杨子%赖关朝%杨燕%林忠宁%唐小江
趙娜%睢罡%李南春%武昕%楊子%賴關朝%楊燕%林忠寧%唐小江
조나%휴강%리남춘%무흔%양자%뢰관조%양연%림충저%당소강
三甲基氯化锡%H(+)K(+)交换ATP酶%基因表达
三甲基氯化錫%H(+)K(+)交換ATP酶%基因錶達
삼갑기록화석%H(+)K(+)교환ATP매%기인표체
trimethyltin chloride%H+-K+-Exchanging ATPase%Gene expression
目的 探索三甲基氯化锡(trimethyltin chloride,TMT)对亚慢性染毒大鼠肾氢钾ATP酶活力、蛋白和基因表达水平的影响.方法 取6周龄雌性SD大鼠55只,随机分成对照、低剂量、中剂量、高剂量和超高剂量组,共5组,每组11只,分别按0、8.20、32.81、131.25、262.50μg·kg-1·d-1的TMT剂量经饮水亚慢性染毒,14周后测定血钾水平,取大鼠肾脏,用定磷法测量氢钾ATP活力,用免疫印迹法测定肾氢钾ATP酶蛋白表达水平,用实时定量-聚合酶链反应法测定肾氢钾ATP酶的mRNA表达水平.结果 与对照组[(6.9±0.3)mmol/L]比较,超高剂量组血钾水平[(5.6±0.4)mmol/L]明显降低,差异有统计学意义(P＜.01).低剂量和超高剂量组氢钾ATP酶活力分别为(4.50±1.45)、(435±-0.72)μmolPi·mgprot-1·h-1,明显低于对照组[(6.55±0.77)μmolPi·mgprot-1·h-1],差异有统计学意义(P＜0.05).与对照组比较,超高剂量组氢钾ATP酶蛋白和mRNA表达水平差异均无统计学意义(P＞0.05).结论 TMT亚慢性接触可抑制氢钾ATP酶的活力,但不抑制该酶的蛋白和基冈表达.
目的 探索三甲基氯化錫(trimethyltin chloride,TMT)對亞慢性染毒大鼠腎氫鉀ATP酶活力、蛋白和基因錶達水平的影響.方法 取6週齡雌性SD大鼠55隻,隨機分成對照、低劑量、中劑量、高劑量和超高劑量組,共5組,每組11隻,分彆按0、8.20、32.81、131.25、262.50μg·kg-1·d-1的TMT劑量經飲水亞慢性染毒,14週後測定血鉀水平,取大鼠腎髒,用定燐法測量氫鉀ATP活力,用免疫印跡法測定腎氫鉀ATP酶蛋白錶達水平,用實時定量-聚閤酶鏈反應法測定腎氫鉀ATP酶的mRNA錶達水平.結果 與對照組[(6.9±0.3)mmol/L]比較,超高劑量組血鉀水平[(5.6±0.4)mmol/L]明顯降低,差異有統計學意義(P＜.01).低劑量和超高劑量組氫鉀ATP酶活力分彆為(4.50±1.45)、(435±-0.72)μmolPi·mgprot-1·h-1,明顯低于對照組[(6.55±0.77)μmolPi·mgprot-1·h-1],差異有統計學意義(P＜0.05).與對照組比較,超高劑量組氫鉀ATP酶蛋白和mRNA錶達水平差異均無統計學意義(P＞0.05).結論 TMT亞慢性接觸可抑製氫鉀ATP酶的活力,但不抑製該酶的蛋白和基岡錶達.
목적 탐색삼갑기록화석(trimethyltin chloride,TMT)대아만성염독대서신경갑ATP매활력、단백화기인표체수평적영향.방법 취6주령자성SD대서55지,수궤분성대조、저제량、중제량、고제량화초고제량조,공5조,매조11지,분별안0、8.20、32.81、131.25、262.50μg·kg-1·d-1적TMT제량경음수아만성염독,14주후측정혈갑수평,취대서신장,용정린법측량경갑ATP활력,용면역인적법측정신경갑ATP매단백표체수평,용실시정량-취합매련반응법측정신경갑ATP매적mRNA표체수평.결과 여대조조[(6.9±0.3)mmol/L]비교,초고제량조혈갑수평[(5.6±0.4)mmol/L]명현강저,차이유통계학의의(P＜.01).저제량화초고제량조경갑ATP매활력분별위(4.50±1.45)、(435±-0.72)μmolPi·mgprot-1·h-1,명현저우대조조[(6.55±0.77)μmolPi·mgprot-1·h-1],차이유통계학의의(P＜0.05).여대조조비교,초고제량조경갑ATP매단백화mRNA표체수평차이균무통계학의의(P＞0.05).결론 TMT아만성접촉가억제경갑ATP매적활력,단불억제해매적단백화기강표체.
Objective To study the activity、protein and gene expression of renal HK-ATPase (HKA) in rats subchronicly exposed to trimethyltin chloride (TMT). Methods In subchronic toxic test (14-week), 55 female SD rats (age, 6 weeks) were divided randomly into 5 groups: control, low, medium, high and super high dosage, respectively, which drank water with TMT of 0, 8.20, 32.81, 131.25 and 262.50 μg·kg-1 ·d-1 for 14 weeks.Then serum K+ levels were measured; the activities of H K-ATPase (HKA) in kidneys were detected by the method of determinenate phosphorus content;Western Blot assay and real-time PCR were used to exam the protein and mRNA expression levels of HKA in kidneys, respectively. Results The serum K + level in super-high dosage group was (5.6±0.4) mmol/L, which was significantly lower than that [(6.9±0.3) mmol/L] in control group (P＜0.01).The HKA enzymatic activity of kidneys in low and super high dosage groups was 4.50±1.45 and 4.55±0.72 μmolPi·mg prot-1h-1, respectively, which were significantly lower than that (6.55±0.77 μmol Pi ·mg prot-1h-1) in control group (P＜0.05). Conclusion When rats were exposed subchronicly to TMT, the renal HKA activity could reduce, but the expression levels of HKA protein and mRNA did not decrease.
