中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2012年
4期
354-357
,共4页
田素青%王俊杰%赵勇%江萍%姜玉良%王皓%孟娜
田素青%王俊傑%趙勇%江萍%薑玉良%王皓%孟娜
전소청%왕준걸%조용%강평%강옥량%왕호%맹나
西妥昔单抗%Tca8113细胞%放射增敏%细胞周期%凋亡
西妥昔單抗%Tca8113細胞%放射增敏%細胞週期%凋亡
서타석단항%Tca8113세포%방사증민%세포주기%조망
Cetuximab%Tca8113%Radiosensitization%Cell cycle%Apoptosis
目的 探讨西妥昔单抗( C225)对人舌癌Tca8113细胞系的放射增敏作用.方法 体外培养人舌癌Tca8113细胞系,C225组用西妥昔单抗(C225) 100 nmol/L处理后,6MVX射线不同剂量(0、2、4、6、8和10 Gy)照射C225组和单纯照射组,照射后用细胞计数法及四甲基偶氮唑盐比色法(MTT)检测细胞增殖率,集落形成实验计算克隆数,采用流式细胞仪检测细胞周期、细胞凋亡.结果 不同照射剂量时C225+照射组的细胞生长抑制率均高于单纯照射组(t=-15.6～-3.0,P ＜0.05)；C225+照射组的放射生物学参数值(D0、Dq、N、SF2)较单纯照射组低.C225+照射组的SER为1.353;C225+照射组的G0/G1期细胞比例在4、6、8 Gy时均高于单纯照射组(t=-7.64、-7.89、-4.78,P＜0.05).随着照射剂量升高,C225+照射组和单纯照射组的细胞早期凋亡率皆先升高后下降,4 Gy时两组差异最大[(7.96±0.36)％和(4.13±0.29)％,t- 12.75,P＜0.01].结论 C225对人舌癌Tca8113细胞系具有放射增敏作用.C225可能通过Go/G.期阻滞和诱导凋亡来增加人舌癌Tca8113细胞系的放射敏感性.
目的 探討西妥昔單抗( C225)對人舌癌Tca8113細胞繫的放射增敏作用.方法 體外培養人舌癌Tca8113細胞繫,C225組用西妥昔單抗(C225) 100 nmol/L處理後,6MVX射線不同劑量(0、2、4、6、8和10 Gy)照射C225組和單純照射組,照射後用細胞計數法及四甲基偶氮唑鹽比色法(MTT)檢測細胞增殖率,集落形成實驗計算剋隆數,採用流式細胞儀檢測細胞週期、細胞凋亡.結果 不同照射劑量時C225+照射組的細胞生長抑製率均高于單純照射組(t=-15.6～-3.0,P ＜0.05)；C225+照射組的放射生物學參數值(D0、Dq、N、SF2)較單純照射組低.C225+照射組的SER為1.353;C225+照射組的G0/G1期細胞比例在4、6、8 Gy時均高于單純照射組(t=-7.64、-7.89、-4.78,P＜0.05).隨著照射劑量升高,C225+照射組和單純照射組的細胞早期凋亡率皆先升高後下降,4 Gy時兩組差異最大[(7.96±0.36)％和(4.13±0.29)％,t- 12.75,P＜0.01].結論 C225對人舌癌Tca8113細胞繫具有放射增敏作用.C225可能通過Go/G.期阻滯和誘導凋亡來增加人舌癌Tca8113細胞繫的放射敏感性.
목적 탐토서타석단항( C225)대인설암Tca8113세포계적방사증민작용.방법 체외배양인설암Tca8113세포계,C225조용서타석단항(C225) 100 nmol/L처리후,6MVX사선불동제량(0、2、4、6、8화10 Gy)조사C225조화단순조사조,조사후용세포계수법급사갑기우담서염비색법(MTT)검측세포증식솔,집락형성실험계산극륭수,채용류식세포의검측세포주기、세포조망.결과 불동조사제량시C225+조사조적세포생장억제솔균고우단순조사조(t=-15.6～-3.0,P ＜0.05)；C225+조사조적방사생물학삼수치(D0、Dq、N、SF2)교단순조사조저.C225+조사조적SER위1.353;C225+조사조적G0/G1기세포비례재4、6、8 Gy시균고우단순조사조(t=-7.64、-7.89、-4.78,P＜0.05).수착조사제량승고,C225+조사조화단순조사조적세포조기조망솔개선승고후하강,4 Gy시량조차이최대[(7.96±0.36)％화(4.13±0.29)％,t- 12.75,P＜0.01].결론 C225대인설암Tca8113세포계구유방사증민작용.C225가능통과Go/G.기조체화유도조망래증가인설암Tca8113세포계적방사민감성.
Objective To investigate the mechanism of radiosensitization by cetuximab (C225) on human tongue cancer Tca8113 cell line in vitro.Methods Tca8113 cell line with and without C225 treatment received 6 MV X-ray irradiation of different doses (0, 2,4,6, 8 and 10 Gy). Cell proliferation,cell-cycle distribution and clonogenic survival were analyzed through cell counting,MTT,colony formation assay,and flow cytometry,respectively.Results After irradiation of different doses,the growth inhibition rates in C225 group were higher than control (t =- 15.6 - -3.0,P＜0.05),the radiobiological parameters (D0,Dq,N,and SF2 ) in C225 group were lower than control so that SER of C225 group was 1.353,and the proportions of G0/G1 cells in C225 group were higher than control ( t =-7.64,-7.89,-4.78,P ＜0.05 ) at 4,6,8 Gy.When the irradiation doses increased,the early phase apoptosis in both groups increased at first and then decreased with the maximum difference at 4 Gy [(7.96±0.36)％ in C225 group and (4.13 ±0.29)％ in control group,t =-12.75,P＜0.01 ].Conclusions C225 has radiosensitization effect on Tca8113 cell line,possible through Go/G1 arrest and induction of apoptosia.
