中国行为医学科学
中國行為醫學科學
중국행위의학과학
2008年
12期
1069-1071
,共3页
郑泰吉%玄光洙%李光昭%崔尚允
鄭泰吉%玄光洙%李光昭%崔尚允
정태길%현광수%리광소%최상윤
抑郁症%去甲肾上腺素能%5-羟色胺能%神经儿
抑鬱癥%去甲腎上腺素能%5-羥色胺能%神經兒
억욱증%거갑신상선소능%5-간색알능%신경인
Depression%Norepinephrinergic%Serotoninergic%Neuron
目的 了解绝望行为模型大鼠脑去甲肾上腺素(NE)能和5-羟色胺(5-HT)能神经元中fos蛋白(Fos)的表达情况.方法 采用不同强度强迫游泳应激造成绝望行为(BD组)模型和单纯强迫游泳模型(对照组)大鼠各10只,在其蓝斑(LC)切片上做抗Fos和抗酪胺酸羟化酶(TH)免疫组化双重染色,在中缝背核(RD)切片上做抗Fos和抗色氨酸羟化酶(TPH)免疫组化双重染色,40倍光镜F观察和计数每组LC中的TH阳性细胞数和TH/Fos 阳性细胞数、RD中的TPH阳性细胞数和5-HT/Fos阳性细胞数,并进行2组之间的比较.结果 与对照组相比,BD组LC的TH阳性细胞胞体大、染色深,TH/Fos阳性细胞数[BD组(8.9±0.7)个,对照组(3.9±0.9)个,P<0.01]明显增加,RD中出现TPH/Fos 阳性细胞[BD组(5.0±0.8)个,对照组0个,P<0.01].结论 中枢NE能神经元代谢增强导致该神经元变性件改变,可能与抑郁症的发病有关.RD巾的5-HT能神经元在正常情况下可能不参与或很少参与生理活动,只有在较强烈的应急状态下才开始被启动.
目的 瞭解絕望行為模型大鼠腦去甲腎上腺素(NE)能和5-羥色胺(5-HT)能神經元中fos蛋白(Fos)的錶達情況.方法 採用不同彊度彊迫遊泳應激造成絕望行為(BD組)模型和單純彊迫遊泳模型(對照組)大鼠各10隻,在其藍斑(LC)切片上做抗Fos和抗酪胺痠羥化酶(TH)免疫組化雙重染色,在中縫揹覈(RD)切片上做抗Fos和抗色氨痠羥化酶(TPH)免疫組化雙重染色,40倍光鏡F觀察和計數每組LC中的TH暘性細胞數和TH/Fos 暘性細胞數、RD中的TPH暘性細胞數和5-HT/Fos暘性細胞數,併進行2組之間的比較.結果 與對照組相比,BD組LC的TH暘性細胞胞體大、染色深,TH/Fos暘性細胞數[BD組(8.9±0.7)箇,對照組(3.9±0.9)箇,P<0.01]明顯增加,RD中齣現TPH/Fos 暘性細胞[BD組(5.0±0.8)箇,對照組0箇,P<0.01].結論 中樞NE能神經元代謝增彊導緻該神經元變性件改變,可能與抑鬱癥的髮病有關.RD巾的5-HT能神經元在正常情況下可能不參與或很少參與生理活動,隻有在較彊烈的應急狀態下纔開始被啟動.
목적 료해절망행위모형대서뇌거갑신상선소(NE)능화5-간색알(5-HT)능신경원중fos단백(Fos)적표체정황.방법 채용불동강도강박유영응격조성절망행위(BD조)모형화단순강박유영모형(대조조)대서각10지,재기람반(LC)절편상주항Fos화항락알산간화매(TH)면역조화쌍중염색,재중봉배핵(RD)절편상주항Fos화항색안산간화매(TPH)면역조화쌍중염색,40배광경F관찰화계수매조LC중적TH양성세포수화TH/Fos 양성세포수、RD중적TPH양성세포수화5-HT/Fos양성세포수,병진행2조지간적비교.결과 여대조조상비,BD조LC적TH양성세포포체대、염색심,TH/Fos양성세포수[BD조(8.9±0.7)개,대조조(3.9±0.9)개,P<0.01]명현증가,RD중출현TPH/Fos 양성세포[BD조(5.0±0.8)개,대조조0개,P<0.01].결론 중추NE능신경원대사증강도치해신경원변성건개변,가능여억욱증적발병유관.RD건적5-HT능신경원재정상정황하가능불삼여혹흔소삼여생리활동,지유재교강렬적응급상태하재개시피계동.
Objective To investigate the expression of fos protein (Fos) in the norepinephrinergic and serotoninergic neurons of behavioral despair rat's brain. Methods Each of 10 behavioral despair (BD) rats and simple forced swimming rats were made by using different intensity forced swimming stress. Each of immunohistochemical dual-stainning of anti-Fos/tyrosine hydroxylase (TH) or anti-Fos/tryptophan hydroxylate (TPH) were carried out on their slices of locus coemleus (LC) or nucleus raphe dorsalis (RD).The number of TH positive cells and TH/Fos positive cells on the LC and TPH positive cells and TPH/Fos positive cells on the RD observed and counted by using 40 times light microscope and compared between two groups. Results In the BD rats,TH-positive cells on the LC were swelling and staiuing were deep and the number of TH/Fos positive cells on the LC[BD group (8.9±0.7),contml group (3.9±O.9),P<0.01]and TPH/Fos-positive cells [BD group (5.0±0.8),control group O,P<0.01] increased significantly compared with the control group.Conclusion The results suggest that the degeneration of central norepinephrinergic neurons which involved increased metabolism may be relative to incidence of depression.The 5-HT neurons of RD in normal circumstances may not participate or rarely participate in physical activities and only was activated in the more strongly state of stress.
