CAJ | 학술논문

目的检测胃癌及慢性萎缩性胃炎、慢性浅表性胃炎患者的P16的表达水平,探讨抑癌基因在胃癌发生中的作用.方法采用Envisoon二部法对93例胃镜下胃黏膜组织石蜡切片滴加P16单克隆抗体并染色.每批染色均设阴性对照,用PBS替代一抗.用已知阳性切片为阳性对照.免疫组化显色判断采用许氏阳性细胞半定量法[2]判定P16的阳性率.结果 P16阳性率:慢性浅表性胃炎组为50%(15/30),萎缩性胃炎组为68.8%(21/32),不典型增生组58%(7/12),胃癌组29%(9/31),所得数据,检验,胃癌组和慢性浅表性胃炎组者比较二者差异无统计学意义(P＞0.01).胃癌和不典型增生组间比较二者差异无统计学意义(P＞0.01).胃癌和萎缩性胃炎组比较二者差异无统计学意义(P＜0.05).结论 P16蛋白在胃癌的发生发展中起着明显的作用,对预后有很高的价值.
목적 검측위암급만성위축성위염、만성천표성위염환자적P16적표체수평,탐토억암기인재위암발생중적작용.방법 채용Envisoon이부법대93례위경하위점막조직석사절편적가P16단극륭항체병염색.매비염색균설음성대조,용PBS체대일항.용이지양성절편위양성대조.면역조화현색판단채용허씨양성세포반정량법[2]판정P16적양성솔.결과 P16양성솔:만성천표성위염조위50%(15/30),위축성위염조위68.8%(21/32),불전형증생조58%(7/12),위암조29%(9/31),소득수거,검험,위암조화만성천표성위염조자비교이자차이무통계학의의(P＞0.01).위암화불전형증생조간비교이자차이무통계학의의(P＞0.01).위암화위축성위염조비교이자차이무통계학의의(P＜0.05).결론 P16단백재위암적발생발전중기착명현적작용,대예후유흔고적개치.
Objective To investigate the expression standard in patients of gastric carcinoma,chronic atrophic gastric and chronic superficial gastric,to discuss the function of carcinoma repressor gene in gastric carcinoma.Methods Adopt by Envision TM staining,the gastric tissues in paraffin-embedded were effected on P16 specific monoclonal antibodies and the other were composed of PBS.P16 protein gene were characterized by semi-quantitative of immunohistochemistry.Results Immunohistochenistry analysis showed that P16 positive rate:50%(15/30)in chronic superficial gastric group,68.8%(21/32)in chronic atrophic gastric group,58% (7/12)in dysplasia group and29%(9/31)in gastric carcinoma group.There was no difference in comparison of gastric(P＞0.01),and there was no difference in comparison of gastric carcinoma and dysplasia(P＞0.01),string difference was found in comparison of gastric carcinoma and chronic atrophic gastric(P＜0.05).Conclusion P16 Protein gene may play an important role in the pathogenesis in gastric carcinoma.