CAJ | 학술논문

目的探讨谷胱甘肽转硫酶(GSTs)基因多态性与湖北汉族人群溃疡性结肠炎(UC)的关系.方法回顾性分析2002年8月至2009年12月武汉大学中南医院、武汉大学人民医院、华中科技大学附属同济医院和协和医院收治的270例湖北汉族UC患者(UC组)及同期623例健康体检者(对照组)的临床资料.根据病变范围将UC患者分为远端UC组(229例)和广泛UC组(41例);根据病变严重程度将UC患者分为轻中度组(237例)和重度组(33例).采用聚合酶链反应检测GSTM1和GSTT1的基因多态性、采用限制性片段长度多态性聚合酶链反应检测GSTP1的基因多态性,并对GSTs基因型进行判定.将含有157 bp片段和480 bp片段者分别定义为GSTM1(+)和GSTT1(+),而无相应的扩增产物者分别定义为GSTM1(-)、GSTT1(-).采用x2检验对数据进行分析.结果 UC组和对照组中GSTM1(-)、GSTT1(-)和GSTP1纯合子突变型基因型(Val/Val)的分布频率分别为70.7%(191/270)、64.8%(175/270)、48.9%(132/270)和41.7%(260/623)、47.2%(294/623)、34.3%(214/623),两组比较,差异有统计学意义(x2=63.404,22.320,25.384,P＜0.05).进一步根据UC临床症状进行分层分析,远端UC组和广泛UC组中GSTT1(-)、GSTP1(Val/Val)的分布频率分别为71.6%(164/229)、57.6%(132/229)和31.7%(13/41)、29.3%(12/41),两组比较,差异有统计学意义(x2=24.528,9.609,P＜0.05).远端UC组与广泛UC组的GSTM1(-)基因型的分布频率分别为65.1%(149/229)和56.1%(23/41),两组比较,差异无统计学意义(x2=1.210,P＞0.05).远端UC组和广泛UC组中GSTT1(-)和GSTP1(Val/Val)的分布频率分别为71.6%(164/229)、31.7%(13/41)和57.6%(132/229)、29.3%(12/41),两组比较,差异有统计学意义(x2=24.528,9.609,P＜0.05).轻中度UC组和重度UC组中GSTM1(-)、GSTT1(-)、GSTP1(Val/Val)分布频率比较,差异无统计学意义(x2=0.623,1.884,3.403,P＞0.05).结论突变的GSTs基因型与湖北汉族人群的UC发生明显相关.GSTs突变基因型可能与UC患者病情严重程度无关. 目的探討穀胱甘肽轉硫酶(GSTs)基因多態性與湖北漢族人群潰瘍性結腸炎(UC)的關繫.方法迴顧性分析2002年8月至2009年12月武漢大學中南醫院、武漢大學人民醫院、華中科技大學附屬同濟醫院和協和醫院收治的270例湖北漢族UC患者(UC組)及同期623例健康體檢者(對照組)的臨床資料.根據病變範圍將UC患者分為遠耑UC組(229例)和廣汎UC組(41例);根據病變嚴重程度將UC患者分為輕中度組(237例)和重度組(33例).採用聚閤酶鏈反應檢測GSTM1和GSTT1的基因多態性、採用限製性片段長度多態性聚閤酶鏈反應檢測GSTP1的基因多態性,併對GSTs基因型進行判定.將含有157 bp片段和480 bp片段者分彆定義為GSTM1(+)和GSTT1(+),而無相應的擴增產物者分彆定義為GSTM1(-)、GSTT1(-).採用x2檢驗對數據進行分析.結果 UC組和對照組中GSTM1(-)、GSTT1(-)和GSTP1純閤子突變型基因型(Val/Val)的分佈頻率分彆為70.7%(191/270)、64.8%(175/270)、48.9%(132/270)和41.7%(260/623)、47.2%(294/623)、34.3%(214/623),兩組比較,差異有統計學意義(x2=63.404,22.320,25.384,P＜0.05).進一步根據UC臨床癥狀進行分層分析,遠耑UC組和廣汎UC組中GSTT1(-)、GSTP1(Val/Val)的分佈頻率分彆為71.6%(164/229)、57.6%(132/229)和31.7%(13/41)、29.3%(12/41),兩組比較,差異有統計學意義(x2=24.528,9.609,P＜0.05).遠耑UC組與廣汎UC組的GSTM1(-)基因型的分佈頻率分彆為65.1%(149/229)和56.1%(23/41),兩組比較,差異無統計學意義(x2=1.210,P＞0.05).遠耑UC組和廣汎UC組中GSTT1(-)和GSTP1(Val/Val)的分佈頻率分彆為71.6%(164/229)、31.7%(13/41)和57.6%(132/229)、29.3%(12/41),兩組比較,差異有統計學意義(x2=24.528,9.609,P＜0.05).輕中度UC組和重度UC組中GSTM1(-)、GSTT1(-)、GSTP1(Val/Val)分佈頻率比較,差異無統計學意義(x2=0.623,1.884,3.403,P＞0.05).結論突變的GSTs基因型與湖北漢族人群的UC髮生明顯相關.GSTs突變基因型可能與UC患者病情嚴重程度無關.
목적 탐토곡광감태전류매(GSTs)기인다태성여호북한족인군궤양성결장염(UC)적관계.방법 회고성분석2002년8월지2009년12월무한대학중남의원、무한대학인민의원、화중과기대학부속동제의원화협화의원수치적270례호북한족UC환자(UC조)급동기623례건강체검자(대조조)적림상자료.근거병변범위장UC환자분위원단UC조(229례)화엄범UC조(41례);근거병변엄중정도장UC환자분위경중도조(237례)화중도조(33례).채용취합매련반응검측GSTM1화GSTT1적기인다태성、채용한제성편단장도다태성취합매련반응검측GSTP1적기인다태성,병대GSTs기인형진행판정.장함유157 bp편단화480 bp편단자분별정의위GSTM1(+)화GSTT1(+),이무상응적확증산물자분별정의위GSTM1(-)、GSTT1(-).채용x2검험대수거진행분석.결과 UC조화대조조중GSTM1(-)、GSTT1(-)화GSTP1순합자돌변형기인형(Val/Val)적분포빈솔분별위70.7%(191/270)、64.8%(175/270)、48.9%(132/270)화41.7%(260/623)、47.2%(294/623)、34.3%(214/623),량조비교,차이유통계학의의(x2=63.404,22.320,25.384,P＜0.05).진일보근거UC림상증상진행분층분석,원단UC조화엄범UC조중GSTT1(-)、GSTP1(Val/Val)적분포빈솔분별위71.6%(164/229)、57.6%(132/229)화31.7%(13/41)、29.3%(12/41),량조비교,차이유통계학의의(x2=24.528,9.609,P＜0.05).원단UC조여엄범UC조적GSTM1(-)기인형적분포빈솔분별위65.1%(149/229)화56.1%(23/41),량조비교,차이무통계학의의(x2=1.210,P＞0.05).원단UC조화엄범UC조중GSTT1(-)화GSTP1(Val/Val)적분포빈솔분별위71.6%(164/229)、31.7%(13/41)화57.6%(132/229)、29.3%(12/41),량조비교,차이유통계학의의(x2=24.528,9.609,P＜0.05).경중도UC조화중도UC조중GSTM1(-)、GSTT1(-)、GSTP1(Val/Val)분포빈솔비교,차이무통계학의의(x2=0.623,1.884,3.403,P＞0.05).결론 돌변적GSTs기인형여호북한족인군적UC발생명현상관.GSTs돌변기인형가능여UC환자병정엄중정도무관.
Objective To investigate the correlation between genetic polymorphisms of glutathione S-transferases (GSTs) and ulcerative colitis (UC) in Hubei Han population. Methods Genetic polymorphisms of GSTM1 and GSTT1 of 270 patients with UC (UC group) who were admitted to the Zhongnan Hospital, People's Hospital of Wuhan University, Tongji Hospital and Union Hospital of Huazhong University of Science and Technology from August 2002 to December 2009 and 623 healthy people ( control group) were detected by restriction fragment length polymorphism-polymerase chain reaction. All UC patients were allocated to distal UC group (n= 229) and extensive UC group (n =41 ) according to the location of the lesions; and all UC patients were also allocated to mild-moderate group (n = 237) and severe group (n = 33 ). The genetic polymorphisms of GSTP1 of these patients and healthy people were detected by polymerase chain reaction. The genotypes of GSTM1, GSTT1 and GSTP1 were also detected. GSTM1 and GSTT1 containing small DNA segments ( 157 bp and 480 bp) were defined as GSTM1 (+) and GSTT1 (+), otherwise, GSTM(-) and GSTT1 (-), respectively. All data were analyzed by chisquare test. Results The frequencies of GSTM1(-), GSTT1(-) and GSTP1 (Val/Val) were 70.7% (191/270),64.8% (175/270) and 48.9% (132/270) in the UC group, and 41.7% (260/623), 47.2% ( 294/623 ) and 34.3% (214/623) in the control group, with a significant difference between the two groups (x2 = 63. 404,22. 320, 25. 384, P ＜0.05 ). The frequencies of GSTT1 (-) and GSTP1 (Val/Val) were 71.6% (164/229) and 57.6% (132/229) in the distal UC group, which were significantly higher than 31.7% (13/41) and 29.3%( 12/41 ) in the extensive UC group ( x2 = 24.528, 9.609, P ＜ 0.05 ). The frequencies of GSTM1 (-) were 65.1%(149/229) in the distal UC group and 56.1% (23/41) in the extensive UC group, with no significant difference between the two groups ( x2 = 1. 210, P ＞ 0.05 ). The frequencies of GSTT1 (-) and GSTP1 ( Val/Val ) were 71.6%(164/229), 31.7% ( 13/41 ) in the distal UC group and 57.6% ( 132/229), 29.3% ( 12/41 ) in the extensive UC group, with a significant difference between the two groups ( x2 = 24. 528, 9. 609, P ＜ 0. 05 ). There was no significant difference in the frequencies of GSTM1 (-), GSTT1 (-), GSTP1 (Val/Val) in the mild-moderate group and the severe group( x2 = 0. 623, 1. 884, 3. 403, P ＞ 0. 05 ). Conclusions Variant genotypes of GSTs are significantly correlated with UC in Hubei Han population. The severity of UC may not be correlated with variant genotypes of GSTs.