CAJ | 학술논문

目的研究鸡眼视网膜多巴胺转运蛋白(dopamine transporter, DAT)的分布及其在实验性近视眼形成中的变化及可能作用。方法　孵化第2天的雏鸡随机分为4组,Ⅰ、Ⅱ、Ⅲ组小鸡单眼分别戴-10D、-20D透镜及半透明眼罩,IV组为正常对照组。3周后测量双眼屈光状态及眼轴长度。一侧大腿肌注125Ⅰ-β-CIT, 二小时后处死小鸡, 摘出眼球, 解剖出全层视网膜组织(Ⅰ及Ⅳ组)或视网膜神经上皮层与视网膜色素上皮层(retinal pigment epithelium ,RPE)分别取下(Ⅱ及Ⅲ组),以β-计数器分别测定各组织标本多巴胺转运蛋白的放射性计数。结果　(1)RPE组织标本检测到的DAT放射性计数明显高于视网膜神经上皮层。(2)实验眼视网膜组织标本DAT放射性计数大于对照眼。结论　(1)视网膜DAT主要分布在RPE。(2)视网膜DAT可能参与形觉剥夺性近视及透镜诱导性近视的形成。这种研究方法为今后深入研究多巴胺系统与实验性近视眼的关系提供了一条新的途径。
목적연구계안시망막다파알전운단백(dopamine transporter, DAT)적분포급기재실험성근시안형성중적변화급가능작용。방법　부화제2천적추계수궤분위4조,Ⅰ、Ⅱ、Ⅲ조소계단안분별대-10D、-20D투경급반투명안조,IV조위정상대조조。3주후측량쌍안굴광상태급안축장도。일측대퇴기주125Ⅰ-β-CIT, 이소시후처사소계, 적출안구, 해부출전층시망막조직(Ⅰ급Ⅳ조)혹시망막신경상피층여시망막색소상피층(retinal pigment epithelium ,RPE)분별취하(Ⅱ급Ⅲ조),이β-계수기분별측정각조직표본다파알전운단백적방사성계수。결과　(1)RPE조직표본검측도적DAT방사성계수명현고우시망막신경상피층。(2)실험안시망막조직표본DAT방사성계수대우대조안。 결론　(1)시망막DAT주요분포재RPE。(2)시망막DAT가능삼여형각박탈성근시급투경유도성근시적형성。저충연구방법위금후심입연구다파알계통여실험성근시안적관계제공료일조신적도경。
Objective To investigate the distribution, changes and a possible role for retinal dopamine transporter (DAT) in experimental myopia in chickens. Methods Two-day-old chickens were divided into four groups. Chicken eyes were fitted with lenses of -10D,-·$20D and translucent goggles unilaterally. Normal eyes were used as controls. After 3 wk, all chickens were given an intramuscular injection of 125 I-β-CIT[2β-carbomethoxy-3β-(4-iodophenyl)tropane] and sacrificed two hours post injection. Retinal pigment epithelium (RPE) and the neural retina were obtained together or RPE was dissected out from the neural retina. Radioactive DAT from each specimen was assayed by γ-counter. Results Retinal DAT was detected in RPE specimens rather than in the neural retina in all eyes. Radioactive DAT in myopic eyes was higher, compared with control eyes. Conclusions Retinal DAT is mainly located in the RPE and may be involved in the formation of lens induced myopia (LIM) and form deprivation myopia (FDM). These methods may provide a new approach for further studying the role of the dopamine system in experimental myopia.