CAJ | 학술논문

目的研究中枢胆碱能神经系统功能引起的外周淋巴系统变化,为临床诊断治疗提供参考指标.方法采用大鼠脑室内微量反复注射喹啉酸(quinolinic acid,QA)造成的学习记忆障碍模型和自然衰老大鼠,测定其学习记忆功能和脑内胆碱能神经系统的变化.结果 QA模型的学习记忆能力明显受损,脑内胆碱能神经标志酶ChAT活性明显下降.脑海马、大脑皮层胆碱能毒蕈碱受体(MR)结合活性分别降低30%和26%;烟碱受体(NR)结合活性分别降低33%和27%,并较老年鼠明显.进一步发现,各组外周脾淋巴细胞膜胆碱能受体活性的改变与脑内变化一致.结论提示外周生物学指标可能间接反映中枢神经功能的变化而成为诊断某些脑疾患的重要参考.
목적연구중추담감능신경계통공능인기적외주림파계통변화,위림상진단치료제공삼고지표.방법채용대서뇌실내미량반복주사규람산(quinolinic acid,QA)조성적학습기억장애모형화자연쇠로대서,측정기학습기억공능화뇌내담감능신경계통적변화.결과 QA모형적학습기억능력명현수손,뇌내담감능신경표지매ChAT활성명현하강.뇌해마、대뇌피층담감능독심감수체(MR)결합활성분별강저30%화26%;연감수체(NR)결합활성분별강저33%화27%,병교노년서명현.진일보발현,각조외주비림파세포막담감능수체활성적개변여뇌내변화일치.결론제시외주생물학지표가능간접반영중추신경공능적변화이성위진단모사뇌질환적중요삼고.
Objective To provide a useful biological index for clinical diagnosis of Alzheimer's disease (AD) by determination the functional changes in the central cholinergic nerve and their effects on the peripheral lymphatic system. Methods The learning and memory impairment model was established through intraventricular injecting quinolinic acid (QA) repeatedly. Results There was a significant decline of cholineacetyltransferase (ChAT) in cerebral cortex and hippocampus after QA injection. The significantly lower binding activities of acetylcholine muscarinic (M) and nicotinic (N) cholinergic receptors in the hippocampus and cortex in the QA group were found as compared with the sham-operated group (P<0.01). Similar changes were found in the binding activities of M-and N-receptors on spleen lymphocytes. Conclusion Certain lesion of the central nervous system can be reflected in peripheral spleen lymphocytes, which may be an important reference to diagnose the changes of the central nervous system.