CAJ | 학술논문

以金线莲野生植株茎段为外植体,在无菌系建立、增殖和生根培养时进行了适宜培养基的筛选试验,结果表明：以MS为诱导培养基建立无菌系效果较好;以MS＋KT 1.0 mg/L（单位下同）＋IAA 0.2 mg/L＋0.15%活性炭能有效增殖,匍匐茎节部白色芽点多,易生成片状丛生芽块;在1/2 MS＋IAA 2.0＋0.15%活性炭的培养基上生根率达95%以上。同时开展了组培苗移栽试验,以泥炭土∶腐殖土∶蛭石=1∶1∶1配比为组培苗移栽适宜基质,在可控的设施环境下或透光度50%左右的林下移栽,成活率达87.6%。
이금선련야생식주경단위외식체,재무균계건립、증식화생근배양시진행료괄의배양기적사선시험,결과표명：이MS위유도배양기건립무균계효과교호;이MS＋KT 1.0 mg/L（단위하동）＋IAA 0.2 mg/L＋0.15%활성탄능유효증식,포복경절부백색아점다,역생성편상총생아괴;재1/2 MS＋IAA 2.0＋0.15%활성탄적배양기상생근솔체95%이상。동시개전료조배묘이재시험,이니탄토∶부식토∶질석=1∶1∶1배비위조배묘이재괄의기질,재가공적설시배경하혹투광도50%좌우적림하이재,성활솔체87.6%。
With the stem segments of wild Anoectochilus roxburghii plants as explants,the experiments on the selection of suitable medium for induction,proliferation and rooting were carried out.The results showed that the suitable induction medium was MS;the proliferation medium was MS＋KT 1.0 mg/L ＋IAA 0.2 mg/L ＋0.15% activated carbon,creeping section part has white buds and with more generate block buds flakes;and the rooting medium was 1/2 MS＋IAA 2.0 mg/L ＋0.15% activated carbon,then the rooting rate can reach above 95%.Meanwhile,the tests of tissue cultural plantlets transplanting were developed,and the results were demonstrated that the mixed material with peat：muck：vermiculite=1∶1∶1 was suited for the transplanting in forests with a controlled facilities environment,or about 50% transmittance,and the survival rate can reach 87.6%.