CAJ | 학술논문

[目的]考察迟钝爱德华氏菌（Edwardsiellatarda）EIB202中大质粒pEIB202在其致病过程中的作用，将质粒pEIB202消除，为开发抗迟钝爱德华氏菌病的安全减毒的活疫苗打下良好基础。【方法]采用同源重组技术以sacB为反向筛选标记消除质粒。[结果】对质粒pEIB202进行序列分析，发现该质粒编码多种抗性基因及部分Ⅵ型分泌系统（T4SS）组分，说明该质粒可能与E．tarda的多重耐药性及致病力相关。质粒消除菌株EIB202Ap丧失氯霉素及四环素抗性，在生长、毒力、胞外蛋白分泌等方面与野生株无显著差异。【结论]pEIB202质粒是造成EIB202多重耐药性的主要原因，在其致病过程中可能并不起直接作用。
[목적]고찰지둔애덕화씨균（Edwardsiellatarda）EIB202중대질립pEIB202재기치병과정중적작용，장질립pEIB202소제，위개발항지둔애덕화씨균병적안전감독적활역묘타하량호기출。【방법]채용동원중조기술이sacB위반향사선표기소제질립。[결과】대질립pEIB202진행서렬분석，발현해질립편마다충항성기인급부분Ⅵ형분비계통（T4SS）조분，설명해질립가능여E．tarda적다중내약성급치병력상관。질립소제균주EIB202Ap상실록매소급사배소항성，재생장、독력、포외단백분비등방면여야생주무현저차이。【결론]pEIB202질립시조성EIB202다중내약성적주요원인，재기치병과정중가능병불기직접작용。
[Objective] The aim of the research was to investigate the function of large plasmid pEIB202 in the pathogenesis of Edwardsiella tarda EIB202 and to eliminate the plasmid pEIB202,so as to lay the foundation for developing safe and live attenu- ated vaccine against E, tarda. [Method] sacB was used as reverse screening marker to eliminate the plasmid by using homologous recombination technique. [Result] The plasmid pEIB202 was sequenced and it was found that the plasmid encoded multiple resistant genes and some components in type IV secretion system（T4SS）,which sug- gested that the plasmid might be related with the multiple drug-resistance and pathogenicity of E. tarda. The plasmid-eliminated strain EIB202Ap lost the resistance to chloramphenicol and tetracycline,but its growth,virulence and secretion of extra- cellular proteins had no significant difference with wild-type plants. [Conclusion] pEIB202 plasmid is the main reason that caused the multi-drug resistance of EIB202 and might have indirect effects in the pathogenesis of EIB202.