现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2011年
23期
254-256
,共3页
张雅晨%姜亚博%周烽明%顾季春%顾福根
張雅晨%薑亞博%週烽明%顧季春%顧福根
장아신%강아박%주봉명%고계춘%고복근
蝴蝶兰%组织培养%快速繁殖%诱导%增殖%生根
蝴蝶蘭%組織培養%快速繁殖%誘導%增殖%生根
호접란%조직배양%쾌속번식%유도%증식%생근
Phalaenopsis amabilis（L.）C.L.Blume%tissue culture%rapid propagation%introduce%multiplication%rooting
以残败花梗为外植体进行组织培养,建立了蝴蝶兰的无菌繁殖体系,研究了培养基中不同种类植物生长调节物质及其浓度比例对休眠芽的萌发、丛生芽的诱导以及试管苗生根的影响,筛选出了最佳培养基组成;研究了活性炭、PVP、温度、暗培养对培养基褐化的影响。结果表明：在1/2 MS＋6-BA 3.0 mg/L＋NAA 0.2 mg/L＋10%香蕉汁＋3%蔗糖＋0.6%琼脂的培养基上休眠芽的诱导效果最好,诱导率可达87.5%;在1/2 MS＋6-BA 6.0 mg/L＋NAA 0.1 mg/L＋10%香蕉汁＋3%蔗糖＋0.6%琼脂的培养基上丛生芽的诱导效果最好,增殖系数可达3.15;在1/2 MS＋NAA 0.5 mg/L＋活性炭1 000 mg/L＋3%蔗糖＋0.6%琼脂的培养基上生根效果最好,生根率可达95%,平均每株生根3.11条左右,炼苗后移植成活率可达90%。
以殘敗花梗為外植體進行組織培養,建立瞭蝴蝶蘭的無菌繁殖體繫,研究瞭培養基中不同種類植物生長調節物質及其濃度比例對休眠芽的萌髮、叢生芽的誘導以及試管苗生根的影響,篩選齣瞭最佳培養基組成;研究瞭活性炭、PVP、溫度、暗培養對培養基褐化的影響。結果錶明：在1/2 MS＋6-BA 3.0 mg/L＋NAA 0.2 mg/L＋10%香蕉汁＋3%蔗糖＋0.6%瓊脂的培養基上休眠芽的誘導效果最好,誘導率可達87.5%;在1/2 MS＋6-BA 6.0 mg/L＋NAA 0.1 mg/L＋10%香蕉汁＋3%蔗糖＋0.6%瓊脂的培養基上叢生芽的誘導效果最好,增殖繫數可達3.15;在1/2 MS＋NAA 0.5 mg/L＋活性炭1 000 mg/L＋3%蔗糖＋0.6%瓊脂的培養基上生根效果最好,生根率可達95%,平均每株生根3.11條左右,煉苗後移植成活率可達90%。
이잔패화경위외식체진행조직배양,건립료호접란적무균번식체계,연구료배양기중불동충류식물생장조절물질급기농도비례대휴면아적맹발、총생아적유도이급시관묘생근적영향,사선출료최가배양기조성;연구료활성탄、PVP、온도、암배양대배양기갈화적영향。결과표명：재1/2 MS＋6-BA 3.0 mg/L＋NAA 0.2 mg/L＋10%향초즙＋3%자당＋0.6%경지적배양기상휴면아적유도효과최호,유도솔가체87.5%;재1/2 MS＋6-BA 6.0 mg/L＋NAA 0.1 mg/L＋10%향초즙＋3%자당＋0.6%경지적배양기상총생아적유도효과최호,증식계수가체3.15;재1/2 MS＋NAA 0.5 mg/L＋활성탄1 000 mg/L＋3%자당＋0.6%경지적배양기상생근효과최호,생근솔가체95%,평균매주생근3.11조좌우,련묘후이식성활솔가체90%。
Flower peduncles of Phalaenopsis amabilis（L.）C.L.Blume were cultured to induce seedlings on medium,then shoots were cultured as explants to produce shoot cluters in vitro.The effects of different plant growth regulators and their concentrations on the germination of dormant buds,induction of clusters buds and vitro rooting were studied to select the best medium.The effects of activated charcoal,PVP,temperature,dark culture on the medium browning were also investigated.The results showed that：the best media inducing dormant buds was 1/2 MS＋6-BA 3.0 mg/L＋NAA 0.2 mg/L＋10% banana juice＋3% sucrose＋0.6% agar,the induction rate of shoots was up to 87.5%.The best media inducing clusters buds was 1/2 MS＋6-BA 6.0 mg/L＋NAA 0.1 mg/L＋10% banana juice＋3% sucrose＋0.6% agar,the multiplication coefficient was up to 3.15.The best media inducing rooting was 1/2 MS＋NAA 0.5 mg/L＋1 000 mg/L activaed charcoal＋3% sucrose＋0.6% agar,the rooting rate reached to 95%,average number of roots was about 3.11 stripes/plant,transplant survival rate was 90% after hardening seedling.
