粮食科技与经济
糧食科技與經濟
양식과기여경제
GRAIN TECHNOLOGY AND ECONOMY
2011年
6期
50-51
,共2页
张晓燕%黄力%倪小英%黄黎慧
張曉燕%黃力%倪小英%黃黎慧
장효연%황력%예소영%황려혜
苯并芘%抽油烟机中油%高效液相色谱法
苯併芘%抽油煙機中油%高效液相色譜法
분병비%추유연궤중유%고효액상색보법
benzo（a）pyrene%the oil from kitchen ventilator%HPLC
采用高效液相色谱法测定抽油烟机中油的苯并芘含量。采用ZORBOX SB-C18色谱柱;流动相：乙腈＋水=88＋12;流速：1.0 mL/min;柱温：26℃;荧光检测器：发射波长406 nm,激发波长384 nm。测得苯并芘含量在（2.1－84）ng/mL,其线性回归方程为Area=1.1172×Amt-0.41622,相关系数r=0.99983。苯并芘回收率为89.0%－100.1%。样品经过处理后,测定抽油烟机中油的苯并芘含量为35μg/kg,严重超过国家限量标准。
採用高效液相色譜法測定抽油煙機中油的苯併芘含量。採用ZORBOX SB-C18色譜柱;流動相：乙腈＋水=88＋12;流速：1.0 mL/min;柱溫：26℃;熒光檢測器：髮射波長406 nm,激髮波長384 nm。測得苯併芘含量在（2.1－84）ng/mL,其線性迴歸方程為Area=1.1172×Amt-0.41622,相關繫數r=0.99983。苯併芘迴收率為89.0%－100.1%。樣品經過處理後,測定抽油煙機中油的苯併芘含量為35μg/kg,嚴重超過國傢限量標準。
채용고효액상색보법측정추유연궤중유적분병비함량。채용ZORBOX SB-C18색보주;류동상：을정＋수=88＋12;류속：1.0 mL/min;주온：26℃;형광검측기：발사파장406 nm,격발파장384 nm。측득분병비함량재（2.1－84）ng/mL,기선성회귀방정위Area=1.1172×Amt-0.41622,상관계수r=0.99983。분병비회수솔위89.0%－100.1%。양품경과처리후,측정추유연궤중유적분병비함량위35μg/kg,엄중초과국가한량표준。
This thesis developed a HPLC method for determinatio n of benzo（a）pyrene in the oil from kitchen ventilator.In this method,the analysis column is revered-phase column of C18（5 m,4.6 mm×250 mmID）,the mobile phase is column.CH3CN∶H2O=88∶12,the flow is 1.0 mL/min,excitation wavelength is 384 nm,emission wavelength is 406 nm,the column temperature is 26 ℃.and the injection volume is 20 L.The limit of detection Was 0.84 g/kg.The linear plots were obtained between 2.1～84 ng/mL.Overall recoveries were betwe en 89.0%～100.1%.In the same mothed,the oil from kitchen ventilator of benzene content is 35 g/kg.and benzene content is serious than National standards.
