CAJ | 학술논문

目的：建立去卵巢大鼠骨质疏松模型，通过采用不同治疗强度脉冲电磁场（PEMFs）干预去卵巢大鼠，观察各组大鼠的股骨骨密度的变化，初步确定治疗骨质疏松的最适的治疗强度。方法：按随机的分组原则用密闭信封法将雌性3月龄sD大鼠50只分为5组：SHAM对照组10只、OVX对照组10只、OVXⅠ组10只、OVXⅡ组10只、OVXⅢ组10只。除SHAM对照组以外，对所有动物按文献方法去势造模。OVXⅠ组、OVXⅡ组和OVXⅢ组三组大鼠每天在频率为8Hz，强度分别为0．77mT、3．82mT和9．87mT的磁场环境中照射40min，共30天。SHAM对照组和OVX对照组不干预。各组动物均在满30天后股动脉放血处死，取血清标本送检血清雌二醇水平，右侧股骨作骨密度的测定。结果：OVX对照组大鼠的股骨骨密度均显著低于其它四组大鼠的股骨骨密度（P〈0．05或P〈O．01）。但SHAM对照组、OVXⅠ组、OVXⅡ组、0VXⅢ组大鼠的股骨骨密度之间的差异无统计学意义（P〉0．05）。说明OVXⅠ组、OVXⅡ组、OVXⅢ组三种磁场强度PEMFs均有显著防止去势大鼠股骨骨密度下降的作用，三组间差异无显著性。结论：PEMFs的磁场强度从0．77mT～9．87mT为有效强度，对于去卵巢大鼠的骨密度丢失具有预防性的治疗作用。
목적：건립거란소대서골질소송모형，통과채용불동치료강도맥충전자장（PEMFs）간예거란소대서，관찰각조대서적고골골밀도적변화，초보학정치료골질소송적최괄적치료강도。방법：안수궤적분조원칙용밀폐신봉법장자성3월령sD대서50지분위5조：SHAM대조조10지、OVX대조조10지、OVXⅠ조10지、OVXⅡ조10지、OVXⅢ조10지。제SHAM대조조이외，대소유동물안문헌방법거세조모。OVXⅠ조、OVXⅡ조화OVXⅢ조삼조대서매천재빈솔위8Hz，강도분별위0．77mT、3．82mT화9．87mT적자장배경중조사40min，공30천。SHAM대조조화OVX대조조불간예。각조동물균재만30천후고동맥방혈처사，취혈청표본송검혈청자이순수평，우측고골작골밀도적측정。결과：OVX대조조대서적고골골밀도균현저저우기타사조대서적고골골밀도（P〈0．05혹P〈O．01）。단SHAM대조조、OVXⅠ조、OVXⅡ조、0VXⅢ조대서적고골골밀도지간적차이무통계학의의（P〉0．05）。설명OVXⅠ조、OVXⅡ조、OVXⅢ조삼충자장강도PEMFs균유현저방지거세대서고골골밀도하강적작용，삼조간차이무현저성。결론：PEMFs적자장강도종0．77mT～9．87mT위유효강도，대우거란소대서적골밀도주실구유예방성적치료작용。
Objective：The purpose of this study was to observe the effect of different treatment intensity of pulsed electromagnetic fields（ PEMFs） on bone mineral density of femur in ovariectomized rats, so as to find out the treatment intensity for the best therapeutic efficacy. Methods： Fifty female SD rats were randomly divided into five groups：（ 1 ） sham - operated control （ no PEMFs treatment）, （ 2 ） ovariectomized control （ no PEMFs treatment ）,（3 ） ovariectomized I （ PEMFs treatment at 8Hz frequency with 0.77mT intensity, 40min daily for 30days）, （4） ovariectomized II （PEMFs treatment at 8Hz frequency with 3.82mT intensity, 40min daily 30 days）, and （5） ovariectomized Ill （PEMFs treatment at 8Hz frequency with 9.87mT intensity, 40min daily 30 days）. Except for 10 rats of the sham - operated control group, a standardized ovariectomy was used in every rat. Estradiol （ E2） of serum and bone mineral density of femur were assessed at 30 days after PEMFs treatment. Results： The bone mineral density of the OVXo control group were significantly lower than the other 4 groups （P 〈 0.05 or P 〈 0.01 ）, but the difference in bone mineral density was not significant among the other 4 groups （ P 〉 0. 05 ）. that means OVX 1 , OVX II , OVX III groups can prevent bone mineral density of femur . Conclusion： 0. 77mT -9.87mT magnetic intensity can prevent bone mineral density of femur ovariectomized rats effectively.