CAJ | 학술논문

利用胰蛋白酶消化法成功启动了松江鲈鳍细胞的原代培养,并通过利用不同培养基、培养温度、pH值对松江鲈鳍细胞进行体外培养实验。实验结果显示：含20%FBS的DMEM/F12（pH=7.2）是松江鲈鳍细胞体外培养的最适培养基,鳍细胞的最适培养温度为24℃。
이용이단백매소화법성공계동료송강로기세포적원대배양,병통과이용불동배양기、배양온도、pH치대송강로기세포진행체외배양실험。실험결과현시：함20%FBS적DMEM/F12（pH=7.2）시송강로기세포체외배양적최괄배양기,기세포적최괄배양온도위24℃。
The primary culture of Trachidermus fasciatus fin cells was successfully launched by trypsin digestion.Furthermore,Trachidermus fasciatus fin cells were cultured in vitro with various culture medium and pH values at different temperatures.The results showed that DMEM / F12（pH = 7.2） containing 20% FBS was the optimal medium for Trachidermus fasciatus fin cells cultivation in vitro and the optimal culture temperature was 24 ℃.