CAJ | 학술논문

罗布麻是中国药典收录的传统中药,本研究提取它的主要有效成分罗布麻总黄酮,采用经典抗抑郁评价模型小鼠强迫游泳实验对罗布麻叶总黄酮进行抗抑郁活性评价。试验中给小鼠罗布麻叶总黄酮提取物25mg/kg、50mg/kg和100mg/kg后,观察罗布麻叶总黄酮对小鼠强迫游泳不动时间的影响,结果显示罗布麻总黄酮提取物能显著缩短小鼠强迫游泳不动时间（P〈0.05）,药效与氟西汀相似,表明罗布麻具有明显的抗抑郁活性。在此基础上,应用皮质酮损伤的PC12细胞模型,采用荧光相对定量RT-PCR法检测罗布麻对皮质酮损伤的PC12细胞中脑内脑源性神经营养因子（BDNF）和环磷酸腺苷反应元件结合蛋白（CREB）表达水平的影响。结果表明,皮质酮处理后PC12细胞中BDNF、CREB基因的表达量最低,罗布麻处理后,表达量显著增加,较处理前增加了近十倍,且呈剂量依赖性。本研究结果提示罗布麻抗抑郁机制可能是通过（AC-cAMP-CREB）信号通路促进BDNF、CREB的基因表达而发挥的。
라포마시중국약전수록적전통중약,본연구제취타적주요유효성분라포마총황동,채용경전항억욱평개모형소서강박유영실험대라포마협총황동진행항억욱활성평개。시험중급소서라포마협총황동제취물25mg/kg、50mg/kg화100mg/kg후,관찰라포마협총황동대소서강박유영불동시간적영향,결과현시라포마총황동제취물능현저축단소서강박유영불동시간（P〈0.05）,약효여불서정상사,표명라포마구유명현적항억욱활성。재차기출상,응용피질동손상적PC12세포모형,채용형광상대정량RT-PCR법검측라포마대피질동손상적PC12세포중뇌내뇌원성신경영양인자（BDNF）화배린산선감반응원건결합단백（CREB）표체수평적영향。결과표명,피질동처리후PC12세포중BDNF、CREB기인적표체량최저,라포마처리후,표체량현저증가,교처리전증가료근십배,차정제량의뢰성。본연구결과제시라포마항억욱궤제가능시통과（AC-cAMP-CREB）신호통로촉진BDNF、CREB적기인표체이발휘적。
Apocynum venetum was a traditional drug which was included by Chinese Pharmacopoeia.We extracted the major active component of Apocynum venetum-flavonoid and investigated its antidepressant effect and corresponding mechanisms based on depression animal models.The antidepressant effect and possible mechanism of flavonoid extract from Apocynum venetum leaves （AV-extract） were observed in forced swimming test of mice,administration of AV extract （25mg/kg,50mg/kg and 100mg/kg,ip） reduced the duration time of immobility significantly （P0.05） suggested that Apocynum venetum have outstanding effect of antidepression,and the drug action of it is similar with fluoxetine.To further search for the antidepressant mechanism of Apocynum venetum,corticosterone damaged PC12 cell was used as a model,meanwhile Taqman quantitative testing technique was used to test the expression of the brain-derived neurotrophic factor and cAMP response element binding protein of PC12 cells in the presence of corticosterone （0.01mmol/L） and flavonoid extract from Apocynum venetum leaves （25μg/mL,50 μg/mL and 100μg/mL） for 48 h.The result showed that the expression level of BDNF,CREB was reduced significantly after treatmented by corticosterone only,but was elevated significantly by Apocynum venetum treatment which was up-regulated approximately 10 times with dose-dependment corresponding to only corticosterone treated.The result showed that the possible mechanism of Apocynum venetum maybe related to elevation the expression of BDNF,CREB through the signal path AC-cAMP-CREB.