CAJ | 학술논문

目的：研究缺氧对HSC基质金属蛋白酶Ⅱ（matrix metalloproteinase-2，MMP-2）表达及活性调节的影响。方法：大鼠HSC体外原代培养，以免疫细胞化学及ELISA法分别检测细胞内MMP-2及基质金属蛋白酶组织抑制因子Ⅱ（Tissue inhibitor of metalloproteinase-2，TIMP-2）的表达，和培养上清液中两者的相对量，并以酶图法测培养上清液MMP-2活力。结果：缺氧培养12h，免疫细胞化学显示MMP-2表达高于对照组(t'＝3.4685，P＜0.05＝，TIMP-2表达则降低(t'＝2.5673，P＜0.05 ＝；培养上清液中MMP-2酶活性低于对照组(t′＝4.270，P＜0.01＝。缺氧6h上清液MMP-2蛋白相对含量增加值高于12h及24h组(t值分别为4.0945、2.4652；P值分别为＜0.001、0.025＝；TIMP-2变化趋势与MMP-2则相反，但各组间差异无显著性。结论缺氧使肝星状细胞MMP-2含量增加，该影响在早期更明显。
목적：연구결양대HSC기질금속단백매Ⅱ（matrix metalloproteinase-2，MMP-2）표체급활성조절적영향。방법：대서HSC체외원대배양，이면역세포화학급ELISA법분별검측세포내MMP-2급기질금속단백매조직억제인자Ⅱ（Tissue inhibitor of metalloproteinase-2，TIMP-2）적표체，화배양상청액중량자적상대량，병이매도법측배양상청액MMP-2활력。 결과：결양배양12h，면역세포화학현시MMP-2표체고우대조조(t'＝3.4685，P＜0.05＝，TIMP-2표체칙강저(t'＝2.5673，P＜0.05 ＝；배양상청액중MMP-2매활성저우대조조(t′＝4.270，P＜0.01＝。결양6h상청액MMP-2단백상대함량증가치고우12h급24h조(t치분별위4.0945、2.4652；P치분별위＜0.001、0.025＝；TIMP-2변화추세여MMP-2칙상반，단각조간차이무현저성。결론 결양사간성상세포MMP-2함량증가，해영향재조기경명현。
Objective: To study the effect of hypoxia on the expression and activity of matrix metalloproteinase-2 (MMP-2) in the hepatic stellate cell (HSC). Methods: The expression of MMP-2 and tissue inhibitor of metalloproteinase-2 (TIMP-2) in cultured rat HSC was detected by immunocytochemistry (ICC). The relative amount of MMP-2 and TIMP-2 in the culture supernatant was assayed by ELISA and the activity of MMP-2 in supernatant was examined by zymography. Results: The expression of MMP-2 was enhanced in hypoxia group as compared with that in control one (t'＝3.4685, P＜0.05), while the expression of TIMP-2 was decreased (t'=2.5673, P<0.05). The activity of MMP-2 was lower in hypoxia group than in control one (t'=4.270, P<0.01). Comparing the varied duration of hypoxia, the relative amount of MMP-2 protein was the highest in 6 h group (6h vs 12h, t=4.0945, P<0.001; 6h vs 24h, t=2.4652, P<0.025), with a contrary curve in TIMP-2. Conclusion: Hypoxia promotes the expression of MMP-2 and inhibits the expression of TIMP-2 in HSC. It is more notable at early stage of hypoxia.