CAJ | 학술논문

首先绘制了蟾蜍脊髓灰质腹角运动神经元池的精确定位图谱,然后分别将浓度为1、0.5、0.1、0.01 mol/L的谷氨酸钠溶液及生理盐水(0.65%NaCl,对照组)微量(0.1 μL)注射到蟾蜍脊髓灰质腹角运动神经元池,采用BL-420F生物机能实验系统记录腓肠肌收缩曲线,以收缩波的上升相持续时间、最大张力、张力变化速率、下降相持续时间为指标对各组腓肠肌的收缩曲线进行比较.结果:4组实验组腓肠肌的收缩形式是不同程度的强直收缩;各组收缩波的上升相持续时间、最大张力、张力变化速率在一定程度上存在谷氨酸钠量效依赖关系,且1mol/L组腓肠肌强直收缩的张力、张力变化速率显著人于其他各组,这可能是由于谷氨酸钠与运动神经元上谷氨酸受体结合的数量不同造成的.
수선회제료섬서척수회질복각운동신경원지적정학정위도보,연후분별장농도위1、0.5、0.1、0.01 mol/L적곡안산납용액급생리염수(0.65%NaCl,대조조)미량(0.1 μL)주사도섬서척수회질복각운동신경원지,채용BL-420F생물궤능실험계통기록비장기수축곡선,이수축파적상승상지속시간、최대장력、장력변화속솔、하강상지속시간위지표대각조비장기적수축곡선진행비교.결과:4조실험조비장기적수축형식시불동정도적강직수축;각조수축파적상승상지속시간、최대장력、장력변화속솔재일정정도상존재곡안산납량효의뢰관계,차1mol/L조비장기강직수축적장력、장력변화속솔현저인우기타각조,저가능시유우곡안산납여운동신경원상곡안산수체결합적수량불동조성적.
A diagram of motor neuron pool of ventral horn of spinal cord gray matter in toad was first delineated. Different concentrations (1, 0.5, 0.1, 0.01 mol/L) of excitatory amino acid L-Glu or physiological saline (0.65% NaCl) were then microinjected into the motor neuron pool in a urethane-anaesthetized toad. The contraction curve of the gastrocnemius was then recorded by the BL-420 Physiological Signal Recording. We took the maximal tension, the duration of rising phase, the velocity of tension variation, and the duration of descending phase as the parameters to study the characteristic of gastrocnemius contractility. It was found that the gastrocnemius contractility of all the 4 groups was tetanus but differed in degree, especially the maximal tension, and velocity of tension variation. In contrast to physiological saline, gastrocnemius contracted by the stimulation of L-Giu, and the contraction parameter showed dose-effect relationships except for the duration of descending phase, which was caused by the combination rate of L-Glu and the receptor.