CAJ | 학술논문

通过电化学沉积将壳聚糖、葡萄糖氧化酶和碳纳米管固定到镀铂金电极上,制备了一种新型葡萄糖生物传感器.探讨了铂的电沉积时间、壳聚糖化学沉积时间、缓冲溶液pH和工作电位等对该牛物传感器的影响.实验结果表明,该生物传感器线性范围为1×10~(-6)1.2×10~(-2)mol/L,相关系数为0.9974,检测限为5.0×10~(-7)mol/L,响应时间≤8 s;血清中的尿酸、抗坏血酸等对葡萄糖的测定无干扰.利用该生物传感器测定了人血清中的葡萄糖,回收率在97%～105%之间.该生物传感器线性范围较宽,灵敏度高,响应迅速,抗干扰能力强,有望成为一种可推广的新型葡萄糖检测器.
통과전화학침적장각취당、포도당양화매화탄납미관고정도도박금전겁상,제비료일충신형포도당생물전감기.탐토료박적전침적시간、각취당화학침적시간、완충용액pH화공작전위등대해우물전감기적영향.실험결과표명,해생물전감기선성범위위1×10~(-6)1.2×10~(-2)mol/L,상관계수위0.9974,검측한위5.0×10~(-7)mol/L,향응시간≤8 s;혈청중적뇨산、항배혈산등대포도당적측정무간우.이용해생물전감기측정료인혈청중적포도당,회수솔재97%～105%지간.해생물전감기선성범위교관,령민도고,향응신속,항간우능력강,유망성위일충가추엄적신형포도당검측기.
A new amperometric biosensor for the quantitative measurement of glucose was reported. The biosensor was based on the immobilization of SWNTs-glucose oxidase-chitosan biocomposite at platinized Au electrode. The effect of Pt deposit time, the content of SWNTs and COx, pH of the buffer solution and the operating potential on the perfomanee of the biosensor was studied. The results indicated that the biosonsor has good reproducibility and stability, high sensitivity and rapid response (within 8s) to the determination of glucose. The linear calibration of the biosensor ranges from 1 × 10~(-6) 1.2 × 10~(-2)mol/L with a detection limit of 5× 10~(-7)mol/L( S/N = 3) and a correlation coefficient of 0.9974 for the detection of glucose. The results also showed that there was no interference from the electro-active interfering species such as ascorbic acid and uric acid when they are at endogenous level in serum. The biosensor was used in the determination of glucose in serum with recoveries ranging from 97% to 105%. The biosensor can be used to detect the glucose rapidly and accurately.