CAJ | 학술논문

[目的]为乌骨鸡黑色素的提取提供科学依据.[方法]用改良酸提法对乌骨鸡鸡皮和鸡肉中黑色素的提取方法进行优化,探索制定出详细、可靠的提取方法.[结果]新鲜肌肉、鸡皮均用剁碎的方法,剁得的鸡肉和鸡皮比较细(不能用绞肉机绞);浸泡时间缩短:用浓度6 mol/L盐酸按40 ml/g比例浸泡,时间8.0 h(原12.0 h);回流时间增加:水浴100 ℃回流1.5 h(原1.0 h);在加热回流后进行快速冷冻,用小匙除去其油块,缩短时间,效果较好.利用该方法提取乌骨鸡黑色素平均提取百分含量为0.347%.[结论]建立了乌骨鸡黑色素改良的提取方法.该方法具有可靠、简单、易操作、重复性好、收得率高的优点.
[목적]위오골계흑색소적제취제공과학의거.[방법]용개량산제법대오골계계피화계육중흑색소적제취방법진행우화,탐색제정출상세、가고적제취방법.[결과]신선기육、계피균용타쇄적방법,타득적계육화계피비교세(불능용교육궤교);침포시간축단:용농도6 mol/L염산안40 ml/g비례침포,시간8.0 h(원12.0 h);회류시간증가:수욕100 ℃회류1.5 h(원1.0 h);재가열회류후진행쾌속냉동,용소시제거기유괴,축단시간,효과교호.이용해방법제취오골계흑색소평균제취백분함량위0.347%.[결론]건립료오골계흑색소개량적제취방법.해방법구유가고、간단、역조작、중복성호、수득솔고적우점.
[Objective] The scientific basis of melanin extraction was provided. [Method] The extraction method of the melanin from Wugu chicken skin and meat was optimized through the improved acid formulation method and the reliable extraction method was explored in detail. [ Results] The fresh muscle and skin were chopped into grinder, which was soaked with 40 ml/g of 6 mol/L hydrochloric acid for 8.0 hours(original 12.0 h), then in water at 100℃ for 1.5 hours (original 1.0 h) and finally, was rapidly frozen. The oil block could be removed. The content of melanin extracted from Wugu chicken with the method was averagely 0.347%. [ Conclusion ] The improved extraction method of melanoma from Wugu chicken was established and the method was reliable, simple, easy -to -use, reproducible and high yield.