CAJ | 학술논문

目的考查血栓闭塞性脉管炎血管壁中人巨细胞病毒的感染情况。方法以人巨细胞病毒即刻早期(IE)基因上游的增强子为扩增区段, 用PCR技术分析了41例血栓闭塞性脉管炎病变血管壁中和14例正常血管壁中人巨细胞病毒的感染情况。RCR产物电泳后观察结果。结果 41例血栓闭塞性脉管炎病变血管壁中有28例(68%)查到人巨细胞病毒DNA的存在，14例正常血管壁中有2例(14%)查到人巨细胞病毒DNA的存在。结论在血栓闭塞性脉管炎病变血管壁中存在人巨细胞病毒的感染，人巨细胞病毒可能与血栓闭塞性脉管炎的致病有关。
목적고사혈전폐새성맥관염혈관벽중인거세포병독적감염정황。방법이인거세포병독즉각조기(IE)기인상유적증강자위확증구단, 용PCR기술분석료41례혈전폐새성맥관염병변혈관벽중화14례정상혈관벽중인거세포병독적감염정황。RCR산물전영후관찰결과。결과 41례혈전폐새성맥관염병변혈관벽중유28례(68%)사도인거세포병독DNA적존재，14례정상혈관벽중유2례(14%)사도인거세포병독DNA적존재。결론재혈전폐새성맥관염병변혈관벽중존재인거세포병독적감염，인거세포병독가능여혈전폐새성맥관염적치병유관。
Objective　 To investigate human cytomegalovirus infection (CI) within the vascular wall of thromboangiitis obliterans (TO).　 Methods　 Vascular specimens from 41 patients with thromboangiitis obliterans and 14 normal controls were analyzed by PCR. The nucleic acid sequence of enhancer located in the upstream of immediate early(IE) gene of cytomegalovirus was used as target region for detection of cytomegalovirus DNA. The PCR products were visualized after electrophoresis. 　Results　 Cytomegalovirus DNA was found in 28 (68%) out of the 41 specimens and only in 2 (14%) out of the 14 normal vessels. 　Conclusions　 The results suggested that there was an evidence of CI in the vascular wall of TO. The association between CI and TO needs further investigation.