麦类作物学报
麥類作物學報
맥류작물학보
JOURNAL OF TRITICEAE CROPS
2008年
1期
11-20
,共10页
张立异%Leroy Philip%Bernard Michel%张庆勤%Sourdille Pierre
張立異%Leroy Philip%Bernard Michel%張慶勤%Sourdille Pierre
장립이%Leroy Philip%Bernard Michel%장경근%Sourdille Pierre
普通小麦%EST-SSRs%多态性%遗传作图
普通小麥%EST-SSRs%多態性%遺傳作圖
보통소맥%EST-SSRs%다태성%유전작도
Bread wheat%EST-SSRs%Polymorphism%Genetic mapping
为了解普通小麦EST来源的微卫星的多样性,从大约1 000个包含微卫星的ESTs中设计了300对引物,研究和评估了它们的多态性水平,并且将多态性标记加入到现有的遗传图谱中.在五种不同类型的重复单元中,三个碱基的重复单元出现最多,占到77%.几乎所有的EST SSRs标记(99.3%)的重复单元都含有G-C碱基对.37.4%的微卫星都是四次重复.对于扩增和多态性,300对引物中有60对没有扩增,21.3%的扩增引物没能产生预期的扩增片断.58%的标记至少在所用8个材料的一个中显示出多态性.W7984和Opata组合表现出最高的多态性水平.大多数普通小麦EST-SSRs标记的重复次数小于10,并且4次重复是最为普遍的.81个新的EST-SSR位点被添加到两个已有的参照遗传图谱中(62个加到ITMI,19个加到CTCS).研究结果表明小麦EST-SSRs标记展示了一些不同于基因组微卫星的特异特征,在标记发展和其他遗传应用中,这就使得它们能够成为一种非常有价值的资源.
為瞭解普通小麥EST來源的微衛星的多樣性,從大約1 000箇包含微衛星的ESTs中設計瞭300對引物,研究和評估瞭它們的多態性水平,併且將多態性標記加入到現有的遺傳圖譜中.在五種不同類型的重複單元中,三箇堿基的重複單元齣現最多,佔到77%.幾乎所有的EST SSRs標記(99.3%)的重複單元都含有G-C堿基對.37.4%的微衛星都是四次重複.對于擴增和多態性,300對引物中有60對沒有擴增,21.3%的擴增引物沒能產生預期的擴增片斷.58%的標記至少在所用8箇材料的一箇中顯示齣多態性.W7984和Opata組閤錶現齣最高的多態性水平.大多數普通小麥EST-SSRs標記的重複次數小于10,併且4次重複是最為普遍的.81箇新的EST-SSR位點被添加到兩箇已有的參照遺傳圖譜中(62箇加到ITMI,19箇加到CTCS).研究結果錶明小麥EST-SSRs標記展示瞭一些不同于基因組微衛星的特異特徵,在標記髮展和其他遺傳應用中,這就使得它們能夠成為一種非常有價值的資源.
위료해보통소맥EST래원적미위성적다양성,종대약1 000개포함미위성적ESTs중설계료300대인물,연구화평고료타문적다태성수평,병차장다태성표기가입도현유적유전도보중.재오충불동류형적중복단원중,삼개감기적중복단원출현최다,점도77%.궤호소유적EST SSRs표기(99.3%)적중복단원도함유G-C감기대.37.4%적미위성도시사차중복.대우확증화다태성,300대인물중유60대몰유확증,21.3%적확증인물몰능산생예기적확증편단.58%적표기지소재소용8개재료적일개중현시출다태성.W7984화Opata조합표현출최고적다태성수평.대다수보통소맥EST-SSRs표기적중복차수소우10,병차4차중복시최위보편적.81개신적EST-SSR위점피첨가도량개이유적삼조유전도보중(62개가도ITMI,19개가도CTCS).연구결과표명소맥EST-SSRs표기전시료일사불동우기인조미위성적특이특정,재표기발전화기타유전응용중,저취사득타문능구성위일충비상유개치적자원.
Recently, EST-derived SSRs has become an attractive alternative to complement existing SSR collections in several important crops. However, the information on the occurrence and composition of different SSRs will be important for using them as a source for marker development and other application. In this study, 300 pairs of primers were designed from about 1000 wheat ESTs containing a microsatellite to investigate diversity and characteristics of EST-derived SSRs in wheat (Triticum aestivum), to assess their polymorphism and to integrate polymorphic markers into the reference genetic maps. Among the five different classes of repeated motif, the trinuleotide repeats were the most frequent (77%). Almost all of EST-SSRs (99.33%) contained G-C basepairs at the repeated motif. The SSRs mainly contained four repeats (37.4%). With respect to amplification and polymorphism,60 of 300 primer pairs failed to amplify and 21.3% of the remaining did not yield fragments of the expected size. 139 (58%) of the 240 markers giving an amplification product showed polymorphism on at least one of the eight cultivars used. The cross W7984×Opata (37.5%) showed the most polymorphic. Most wheat EST-SSRs were shorter than 10 repeat units and four units were most frequent.Eighty one new EST-SSR loci were integrated to the two reference genetic maps currently used in the lab (62 on ITMI and 19 on CtCS maps). Our results showed that wheat EST-SSRs markers exhibited some interesting features, different from those of genomic SSRs. This makes them highly valuable as a source for marker development as well as other applications.