植物研究
植物研究
식물연구
BULLETIN OF BOTANICAL RESEARCH
2006年
2期
201-205
,共5页
廊坊杨%芽再生%根再生%愈伤组织%植株再生
廊坊楊%芽再生%根再生%愈傷組織%植株再生
랑방양%아재생%근재생%유상조직%식주재생
Populus langfangensis 3%shooting%rooting%regeneration%callus formation
对杂交杨树新品种廊坊杨3号(Populus langfangensis 3,(P. deltoides ("Shan Hai Guan")×((P. simonii × pyramidalys)12 × Ulmus pumila) 的离体叶片和茎段在附加BA、NAA、IBA和2,4-D的MS培养基上的直接和间接的器官分化、愈伤组织形成和植株再生进行了研究.叶柄和叶片最容易在叶脉处直接诱导生芽.从叶片直接诱导生芽的激素条件为1～2 mg·L-1 BA和0.5 mg·L-1 IBA,最高生芽率可达90%.2,4-D促进愈伤组织的形成.由愈伤组织诱导生芽的激素条件为0.3～0.5 mg·L-1 BA 和 0.02 mg·L-1 IBA或NAA,生芽率达76%.较好的生根条件为0.1 mg·L-1 BA和0.2～0.5 mg·L-1 IBA,生根率可达67%.以上再生条件为廊坊杨3号的转基因育种和无性快繁技术提供了可能.
對雜交楊樹新品種廊坊楊3號(Populus langfangensis 3,(P. deltoides ("Shan Hai Guan")×((P. simonii × pyramidalys)12 × Ulmus pumila) 的離體葉片和莖段在附加BA、NAA、IBA和2,4-D的MS培養基上的直接和間接的器官分化、愈傷組織形成和植株再生進行瞭研究.葉柄和葉片最容易在葉脈處直接誘導生芽.從葉片直接誘導生芽的激素條件為1～2 mg·L-1 BA和0.5 mg·L-1 IBA,最高生芽率可達90%.2,4-D促進愈傷組織的形成.由愈傷組織誘導生芽的激素條件為0.3～0.5 mg·L-1 BA 和 0.02 mg·L-1 IBA或NAA,生芽率達76%.較好的生根條件為0.1 mg·L-1 BA和0.2～0.5 mg·L-1 IBA,生根率可達67%.以上再生條件為廊坊楊3號的轉基因育種和無性快繁技術提供瞭可能.
대잡교양수신품충랑방양3호(Populus langfangensis 3,(P. deltoides ("Shan Hai Guan")×((P. simonii × pyramidalys)12 × Ulmus pumila) 적리체협편화경단재부가BA、NAA、IBA화2,4-D적MS배양기상적직접화간접적기관분화、유상조직형성화식주재생진행료연구.협병화협편최용역재협맥처직접유도생아.종협편직접유도생아적격소조건위1～2 mg·L-1 BA화0.5 mg·L-1 IBA,최고생아솔가체90%.2,4-D촉진유상조직적형성.유유상조직유도생아적격소조건위0.3～0.5 mg·L-1 BA 화 0.02 mg·L-1 IBA혹NAA,생아솔체76%.교호적생근조건위0.1 mg·L-1 BA화0.2～0.5 mg·L-1 IBA,생근솔가체67%.이상재생조건위랑방양3호적전기인육충화무성쾌번기술제공료가능.
The direct and indirect induction of organ differentiation, callus formation and plantlet regeneration from the leaf and stem explants of the hybrid Populus langfangensis 3 (P. deltoides ("Shan Hai Guan")×((P.simonii × pyramidalys)12×Ulmus pumila) were investigated in-vitro on Murashige and Skoog (MS) medium supplemented with various concentrations of benzylaminopurine (BA), indole 3-butyric acid (IBA), 1-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D).The petiole and leaf explant was easier for direct shoot regeneration along the veins at cutting sites. Direct shooting efficiency was related to both BA concentration and BA/IBA ratio. Efficient and rapid shooting from the leaf and stem explants was achieved on MS medium containing 1~2 mg·L-1 BA and 0.5 mg·L-1 IBA. Maximum shooting frequency reached 90%. 2,4-D promoted the callus induction of P. langfangensis 3. The shooting based on callus was obtained on MS medium supplemented with 0.3~0.5 mg·L-1 BA and 0.02 mg·L-1 IBA or NAA and the corresponding shooting frequency was 76%. Rooting based on callus succeeded on 0.1 mg·L-1 BA and 0.2~0.5 mg·L-1 IBA, yielding rooting frequency 67%.These regeneration conditions could further be used for micropropagation and transformation of P.langfangensis 3.
