CAJ | 학술논문

研究了庚型肝炎病毒E2(HGV E2)基因片段作为DNA疫苗的可行性.将来自于质粒pThioHis-E2编码HGV E2的基因片段(559bp)亚克隆到质粒pCMV-S中,使之和HBsAg基因位于同一阅读框,形成重组质粒pCMV-S-E2.用纯化的质pCMV-S-E2 DNA注射到昆明小鼠后腿四头肌中来免疫小鼠,同进用pCMV-S作为对照.间隔14天再加强一次免疫.在加强免疫后8天眼眶取血.用E2-GST融合蛋白作为固定化抗原,通过ELISA检测受试小鼠的体液免疫应答.结果表明,用质粒pCMV-S-E DNA免疫的小鼠可以产生很强的体液免疫应答.
연구료경형간염병독E2(HGV E2)기인편단작위DNA역묘적가행성.장래자우질립pThioHis-E2편마HGV E2적기인편단(559bp)아극륭도질립pCMV-S중,사지화HBsAg기인위우동일열독광,형성중조질립pCMV-S-E2.용순화적질pCMV-S-E2 DNA주사도곤명소서후퇴사두기중래면역소서,동진용pCMV-S작위대조.간격14천재가강일차면역.재가강면역후8천안광취혈.용E2-GST융합단백작위고정화항원,통과ELISA검측수시소서적체액면역응답.결과표명,용질립pCMV-S-E DNA면역적소서가이산생흔강적체액면역응답.
In order to study the feasibility of E2 gene fragment of hepatitis virus G(HGV)as a component of DNA vaccine against the hepatitis virus G infection,a 559bp DNA fragment encoding HGV E2 was cloned into plasmid pCMV-S from pThioHis-E2 in the same reading frame with HBsAg gene to form a recombinant plasmid named pCMV-S-E2.BALB/c mice of Kunming strain were immunized with purified plasmid DNA of pCMV-S-E2 by intra-muscularly inoculation.The immunizations were boosted twice at an interval of 14 days.The whole blood was collected from mice orbit on the day-8 after the last boost.Mice sera were screened by ELISA to determine the humoral immune response using E2-GST fusion protein as the immobilized antigen and the sera from mice immunized with pCMVS as control.The result indicated that the immunization with plasmid DNA of pCMV-S-E2 could induce quite strong humoral immune response.