CAJ | 학술논문

目的探讨氦氖激光诱导瘢痕成纤维细胞凋亡的分子机制.方法将培养瘢痕成纤维细胞随机分为转染组、转染对照组和非转染组,转染组细胞与绿色荧光蛋白 (GFP)质粒 cDNA、含目的基因片段的表达载体质粒 pcDNA3-FADD-DNcDNA共转染,转染对照组细胞与 GFP质粒 cDNA、空载体质粒 pcDNA3共转染,非转染组细胞没有进行转染,转染后 24 h分别对各组细胞进行氦氖激光照射( 100 mW/cm2照射 30 min), 1次 /d,连续照射 3 d,然后采用 TUNEL技术检测细胞凋亡.结果转染组、转染对照组与非转染组细胞凋亡率分别为 9.27% ,15.73%和 12.87%,转染组细胞凋亡率明显小于转染对照组 (q=4.55, P< 0.01)和非转染组( q=4.14, P< 0.05).结论 FADD-DN基因转染能减少氦氖激光诱导的瘢痕成纤维细胞凋亡, FADD可能是氦氖激光激活死亡信号途径的基本元件之一.
목적탐토양내격광유도반흔성섬유세포조망적분자궤제.방법장배양반흔성섬유세포수궤분위전염조、전염대조조화비전염조,전염조세포여록색형광단백 (GFP)질립 cDNA、함목적기인편단적표체재체질립 pcDNA3-FADD-DNcDNA공전염,전염대조조세포여 GFP질립 cDNA、공재체질립 pcDNA3공전염,비전염조세포몰유진행전염,전염후 24 h분별대각조세포진행양내격광조사( 100 mW/cm2조사 30 min), 1차 /d,련속조사 3 d,연후채용 TUNEL기술검측세포조망.결과전염조、전염대조조여비전염조세포조망솔분별위 9.27% ,15.73%화 12.87%,전염조세포조망솔명현소우전염대조조 (q=4.55, P< 0.01)화비전염조( q=4.14, P< 0.05).결론 FADD-DN기인전염능감소양내격광유도적반흔성섬유세포조망, FADD가능시양내격광격활사망신호도경적기본원건지일.
Aim To explore the molecular mechanism of He-Ne laser-inducing apoptosis of scar fibroblasts in vitro.Methods These fibroblasts-devired from hypertrophic scar (HS) were cultured and were divided into three groups:transfection group (TG),control transfection group (CTG) and non-transfection group (NTG).The fibroblasts in TG were co- transfected with GFP (green fluorescent protein) cDNA and pcDNA3-FADD-DN. Scar fibroblasts in CTG were cotransfected with GFP cDNA and pcDNA3. The fibroblasts in NTG was't transfected. Scar fibroblasts of each group were irradiated with He-Ne laser (100 mW/cm2 for 30 minutes) at 24 hours after transfection, once a day for 3 consecutive days.Apoptosis of cell was then examined by TUNEL technique. Results The percent of apoptosis in TG was 9.27% and that of apoptosis was 15.73% in CTG, and 12.87% in NTG.The percent of apoptosis in TG was less than in CTG (q=4.55,P< 0.01) and NTG(q=4.14,P< 0.05) respectively.Conclusion FADD-DN gene transfection can depress He-Ne laser-inducing apoptosis of scar fibroblasts.FADD may be an essential component of the He-Ne laser-activited cell death pathway.