CAJ | 학술논문

目的动态研究哮喘患儿血浆及哮喘豚鼠血液、支气管肺泡灌洗液(BALF)和肺组织中P物质(SP)含量及SP mRNA表达变化,探讨SP与小儿哮喘发病机制的关系.方法用放射免疫方法,动态测定35例不同严重程度哮喘患儿急性发作期及其临床症状缓解期的血浆SP含量变化;并在豚鼠哮喘模型上同期进行了不同严重程度哮喘豚鼠血液、BALF和肺组织中SP含量的对比研究,利用RT-PCR方法研究肺组织SP mRNA表达变化.结果①哮喘患儿急性发作期血浆SP含量较其自身症状缓解期及正常对照组均明显增高,差异有显著性(P＜0.01);症状缓解期血浆SP含量较正常对照组差异无显著性(P＞0.05);②重症哮喘急性发作期血浆SP含量,较轻、中度哮喘急性发作期的含量显著增高,差异有显著性(P＜0.05);③哮喘豚鼠血液、BALF和肺组织中SP含量较正常豚鼠均显著增高(P＜0.01),反复诱喘豚鼠各组织中SP含量均显著高于诱喘一次豚鼠(P＜0.05);④哮喘豚鼠肺组织内SPmRNA表达较正常组显著增高,反复诱喘豚鼠SPmRNA表达显著高于诱喘一次的豚鼠(P＜0.01).结论哮喘患儿及哮喘豚鼠SP含量增加,哮喘豚鼠肺组织中SPmRNA的表达明显上调,均与病情呈显著正相关.SP与小儿哮喘关系密切,可能参与了小儿哮喘的发病机制.
목적동태연구효천환인혈장급효천돈서혈액、지기관폐포관세액(BALF)화폐조직중P물질(SP)함량급SP mRNA표체변화,탐토SP여소인효천발병궤제적관계.방법용방사면역방법,동태측정35례불동엄중정도효천환인급성발작기급기림상증상완해기적혈장SP함량변화;병재돈서효천모형상동기진행료불동엄중정도효천돈서혈액、BALF화폐조직중SP함량적대비연구,이용RT-PCR방법연구폐조직SP mRNA표체변화.결과①효천환인급성발작기혈장SP함량교기자신증상완해기급정상대조조균명현증고,차이유현저성(P＜0.01);증상완해기혈장SP함량교정상대조조차이무현저성(P＞0.05);②중증효천급성발작기혈장SP함량,교경、중도효천급성발작기적함량현저증고,차이유현저성(P＜0.05);③효천돈서혈액、BALF화폐조직중SP함량교정상돈서균현저증고(P＜0.01),반복유천돈서각조직중SP함량균현저고우유천일차돈서(P＜0.05);④효천돈서폐조직내SPmRNA표체교정상조현저증고,반복유천돈서SPmRNA표체현저고우유천일차적돈서(P＜0.01).결론효천환인급효천돈서SP함량증가,효천돈서폐조직중SPmRNA적표체명현상조,균여병정정현저정상관.SP여소인효천관계밀절,가능삼여료소인효천적발병궤제.
Objective To study the changes of substance P (SP) content in plasma of asthmatic children and in theblood, bronchoalveolar lavage fluid (BALF) and lung tissues of asthmatic guinea pigs and SP mRNA expression, and toinvestigate the relationship between SP and asthma of children. Methods We determined the contents of plasma SP bythe method of immunoassay at the stage of acute attack and clinical remission in 35 children with asthma. On the basis ofasthma model of guinea pigs, we examined the contents of SP in the blood, BALF and lungs. The expression of Spthat of the stage of clinical remission and normal controls ( P ＜0.01). At the stage of clinical remission, the differencethe blood, BALF and lungs were higher than those of normal ones ( P ＜0.01), and the expressions of SP mRNA inlungs were higher ( P ＜0.01). In repeatedly attack group, the SP content of guinea pigs was higher than that of asthmagroup ( P ＜ 0.05), and the expression of SP mRNA in lungs was also higher ( P ＜ 0.01 ). Conclusions SP levelsincreased in asthmatic children and asthmatic guinea pigs, and the expression of SP mRNA was up-regulated in the lungsof asthmatic guinea pigs. They were positive related to the severity of asthma. So SP has close relationship with asthmaand may play a role in the mechanism of asthma.