CAJ | 학술논문

用氯仿-甲醇法测定了广州海鲜市场上棕带仙女蛤、波纹巴非蛤、文蛤、栉孔扇贝和园华扇贝等5种贝类的脂肪含量，并用GC-MS法测定了它们的脂肪酸组成。5种贝类鉴定出的脂肪酸都在99%(质量分数)以上。它们的脂肪含量都大于1%(质量分数)，园华扇贝的脂肪含量最高。它们的ω-3多不饱和脂肪酸与ω-6多不饱和脂肪酸含量的比值基本上都大于2。两种扇贝的廿碳五烯酸(EPA)和廿二碳六烯酸(DHA)含量都比较高。分析结果表明，园华扇贝不仅脂肪含量高，而且EPA与DHA的含量也比较高，是EPA和DHA理想的提取原料。
용록방-갑순법측정료엄주해선시장상종대선녀합、파문파비합、문합、즐공선패화완화선패등5충패류적지방함량，병용GC-MS법측정료타문적지방산조성。5충패류감정출적지방산도재99%(질량분수)이상。타문적지방함량도대우1%(질량분수)，완화선패적지방함량최고。타문적ω-3다불포화지방산여ω-6다불포화지방산함량적비치기본상도대우2。량충선패적입탄오희산(EPA)화입이탄륙희산(DHA)함량도비교고。분석결과표명，완화선패불부지방함량고，이차EPA여DHA적함량야비교고，시EPA화DHA이상적제취원료。
The contents of fat and fatty acids in Callista erycina (Linnaeus), Paphia (Paratapes) undulata (Born), Meretrix meretrix (Linnaeus), Chlamys farreri (Jones et Preston) and Patinopecten yessoensis (Jay) were studied． Fat was extracted with Bligh & Dyer (B&D) method． The lipid classes were transesterified with potassium hydroxide in methanol． Fatty acid methyl esters (FAMEs) were assayed with GC-MS and polar capillary column (HP-INNOWax 30 m×0．25 mm i.d.×0．25 μm)． GC injector temperature was 220 ℃． The column temperature was programmed from 150 ℃(1 min) to 200 ℃ at 10 ℃/min and then from 200 ℃ to 250 ℃ at 2 ℃/min． FAMEs were identified by MS library, and part by their standards． Total identified fatty acids were over 99% for all samples． Fat contents of them were all over 1% by wet samples． And ratios between ω-3PUFA and ω-6PUFA were above 2 by and large． Patinopecten yessoensis (Jay) contains more fat and the valuable fatty acids, EPA and DHA． It is suitable to use it as the source of EPA and DHA．