CAJ | 학술논문

木聚糖酶产生菌黑曲霉An-1经紫外线和硫酸二乙酯（DES）处理后，采用显色平皿法及液体培养筛选，得到一产木聚糖酶活力较高的菌株An-1.15,其产酶能力比An-1提高90%。同时研究了周期变压操作对固态发酵过程中黑曲霉产酶的影响。当变压范围为0～0.15 MPa，选取适当变压周期时，黑曲霉木聚糖酶酶活以干料计高达3 690 IUg-1，比静止固态发酵高38%。
목취당매산생균흑곡매An-1경자외선화류산이을지（DES）처리후，채용현색평명법급액체배양사선，득도일산목취당매활력교고적균주An-1.15,기산매능력비An-1제고90%。동시연구료주기변압조작대고태발효과정중흑곡매산매적영향。당변압범위위0～0.15 MPa，선취괄당변압주기시，흑곡매목취당매매활이간료계고체3 690 IUg-1，비정지고태발효고38%。
A mutant strain An-1.15 was obtained with the screening of a colour plate method and liquid state fermentation after Aspergillus niger An-1 producing xylanase was treated with UV and DES. The xylanase of An-1.15 increased by 90%. The influence of periodic air pressure oscillation on xylanase activities was investigated during the course of solid state fermentation of An-1.15. With the air pressure amplitude of 0～0.15 MPa and proper oscillation period, the xylanase activity was as high as 3 690 IU/g dry medium, which was 38% more than xylanase activity of static solid state fermentation.