CAJ | 학술논문

从海域沉积物中富集分离出以芘作为唯一碳源和能源的海洋微生物，以ST4富集培养的混合微生物作为研究对象；该海洋混合菌株能利用菲（Phe）、芘（Pyr）、荧蒽（Fla）等多种多环芳烃；在不同浓度的芘的降解中，当芘的浓度为50mg/dm3时，其生长水平和降解速率最高；当芘的浓度为200mg/dm3时，其生长受到抑制，芘几乎不能被降解．外加营养盐酵母浸出液和葡萄糖促进降解微生物的生长，提高降解速率．研究表明了海洋微生物在多环芳烃污染环境的生物修复应用前景．
종해역침적물중부집분리출이비작위유일탄원화능원적해양미생물，이ST4부집배양적혼합미생물작위연구대상；해해양혼합균주능이용비（Phe）、비（Pyr）、형은（Fla）등다충다배방경；재불동농도적비적강해중，당비적농도위50mg/dm3시，기생장수평화강해속솔최고；당비적농도위200mg/dm3시，기생장수도억제，비궤호불능피강해．외가영양염효모침출액화포도당촉진강해미생물적생장，제고강해속솔．연구표명료해양미생물재다배방경오염배경적생물수복응용전경．
The goal of this paper is to characterize microorganisms (bacteria) isolated from sediment samples contaminated with polycyclic aromatic hydrocarbon(PAHs). ST4——pyrene enriched microorganism were studied. They can use pyrene, phenanthrene, fluoranthene as the only source of carbon and energy. Cells of the microorganism were inoculated in mineral salt medium(MSM) at 10,25,50,100,200 mg/dm3 pyrene in acetone solution. The highest optical density (OD600) and the highest degradation rate were obtained at 50 mg/dm3. Additional nutrients have evident effect on the growth of mixed culture.