CAJ | 학술논문

用根癌农杆菌侵染栝楼（Trichosanthes kirilowiiMaxim.）无菌苗后，获得其冠瘿组织；栝楼冠瘿组织经除菌后能在无激素的MS培养基上良好生长，并合成冠瘿碱，表明Ti质粒转化成功。栝楼冠瘿组织中最高蛋白含量为130.6mg/g(鲜重)。经SDS－PAGE检测其含有的蛋白种类与栝楼根含有的基本一样。研究表明，利用栝楼冠瘿组织作为培养系统生产天花粉蛋白有很好的开发前景。
용근암농간균침염괄루（Trichosanthes kirilowiiMaxim.）무균묘후，획득기관영조직；괄루관영조직경제균후능재무격소적MS배양기상량호생장，병합성관영감，표명Ti질립전화성공。괄루관영조직중최고단백함량위130.6mg/g(선중)。경SDS－PAGE검측기함유적단백충류여괄루근함유적기본일양。연구표명，이용괄루관영조직작위배양계통생산천화분단백유흔호적개발전경。
Transformed crown tissue cultures from Trichosanthes kirilowiiMaxim. were established by Ti plasmid in C58 strains. The crown issues grew well in free- hormone MS medium . Nopaline was inspected in the crown gall and this data showed that T-DNA of Ti plasmid had been transformed successfully .The content of proteins in the tissue was about 130.6mg/g (fresh weight)which was inspected by compound-color methods and its TCN-protein was inspected by SDS-PAGE analysis. The study showed that the production of TCN proteins from crown tissue cultures may be developed in prospect.