湖南医科大学学报
湖南醫科大學學報
호남의과대학학보
BULLETIN OF HUNAN MEDICAL UNIVERSITY
2001年
1期
26-30
,共5页
贺双腾%黎杏群%张花先%罗团连%何泽云%何纲%李家邦%梁清华
賀雙騰%黎杏群%張花先%囉糰連%何澤雲%何綱%李傢邦%樑清華
하쌍등%려행군%장화선%라단련%하택운%하강%리가방%량청화
神经生长因子%白细胞介素-1%细胞,培养的%脑溢安
神經生長因子%白細胞介素-1%細胞,培養的%腦溢安
신경생장인자%백세포개소-1%세포,배양적%뇌일안
目的:观察脑溢安对体外培养神经元血红蛋白损伤后的保护作用及神经生长因子与白细胞介素-1表达的影响。方法:以50μmol.L-1血红蛋白造成培养海马神经元损伤,运用活细胞计数、Northern杂交、酶联免疫检测神经元存活数量、神经生长因子与白细胞介素-1mRNA及其蛋白表达水平。结果:体外培养大鼠海马神经元在含2%牛血清的培养液中可存活5个月以上。加入50μmol.L-1血红蛋白培养24h神经元死亡率为38.5%,同时加入脑溢安药液能显著降低神经元死亡率。血红蛋白可引起培养神经元白细胞介素-1mRNA与蛋白表达水平显著升高及神经生长因子的短暂升高,脑溢安能降低白细胞介素-1表达,维持神经生长因子持续表达。结论:脑溢安对血红蛋白损伤神经元具有保护作用,其机理与调节神经元白细胞素-1、神经生长因子表达有关。
目的:觀察腦溢安對體外培養神經元血紅蛋白損傷後的保護作用及神經生長因子與白細胞介素-1錶達的影響。方法:以50μmol.L-1血紅蛋白造成培養海馬神經元損傷,運用活細胞計數、Northern雜交、酶聯免疫檢測神經元存活數量、神經生長因子與白細胞介素-1mRNA及其蛋白錶達水平。結果:體外培養大鼠海馬神經元在含2%牛血清的培養液中可存活5箇月以上。加入50μmol.L-1血紅蛋白培養24h神經元死亡率為38.5%,同時加入腦溢安藥液能顯著降低神經元死亡率。血紅蛋白可引起培養神經元白細胞介素-1mRNA與蛋白錶達水平顯著升高及神經生長因子的短暫升高,腦溢安能降低白細胞介素-1錶達,維持神經生長因子持續錶達。結論:腦溢安對血紅蛋白損傷神經元具有保護作用,其機理與調節神經元白細胞素-1、神經生長因子錶達有關。
목적:관찰뇌일안대체외배양신경원혈홍단백손상후적보호작용급신경생장인자여백세포개소-1표체적영향。방법:이50μmol.L-1혈홍단백조성배양해마신경원손상,운용활세포계수、Northern잡교、매련면역검측신경원존활수량、신경생장인자여백세포개소-1mRNA급기단백표체수평。결과:체외배양대서해마신경원재함2%우혈청적배양액중가존활5개월이상。가입50μmol.L-1혈홍단백배양24h신경원사망솔위38.5%,동시가입뇌일안약액능현저강저신경원사망솔。혈홍단백가인기배양신경원백세포개소-1mRNA여단백표체수평현저승고급신경생장인자적단잠승고,뇌일안능강저백세포개소-1표체,유지신경생장인자지속표체。결론:뇌일안대혈홍단백손상신경원구유보호작용,기궤리여조절신경원백세포소-1、신경생장인자표체유관。
Objective: To investigate the effects of Nao-yi-an (NYA) onprotecting the cultured hippocampal neurons against the injury induced by hemoglobin (Hb) and modulating the expressions of nerve growth factor (NGF) and interleukin-1β(IL-1β) following Hb injury. Methods: The experimental techniques of neuronal culture, alive cells count, Northern blotting, and enzymelinked immunosorbent assay (ELISA) were employed to detect the survival rate of cultured neurons, the expression level of NGF and IL-1β following Hb injury in cultured hippocampal neurons. Results: ① The hippocampal neurons survived for more than 5 months in Dulbecco's modified Eagle medium (DMEM) containing 2% bovine serum. ② 38.5% cultured neurons were dead in Lock's solution with 5 mmol.L-1 glucose containing 50μmol.L-1 Hb. The rate of survival neurons was significantly increased by addition of decoction of NYA. ③ The expression of IL-1β was markedly increased at 1h, 3h, 6h, and 12h after Hb injury. The expression of NGF was temporarily increased. ④ The levels of IL-1β mRNA and protein were significantly decreased in NYA treated group, while the expression of NGF mRNA and protein was not reduced. Conclusion: NYA has the effects on increasing the neuronal survival, decreasing the expression of IL-1β, and maintaining the expression of NGF following Hb injury in cultured neurons.