CAJ | 학술논문

柚类是柑桔中的一类重要品种,在我国有广泛的分布.近年来,在福建等产区发现柚类病树日趋增多,其病状为叶片斑驳、小果、植株矮化,暂称为叶片斑驳病.本病能通过组织嫁接从病柚树传染到柚健苗和芦柑健苗.在电镜下观察,其病原菌体的形态和细胞壁构造与柑桔黄龙病的病原(Liberobacter asiaticum)十分相似.应用柑桔黄龙病病原DNA的特异性引物进行PCR试验产生的PCR 产物,其分子量与柑桔黄龙病病原DNA PCR 产物的基本相同,为563 bp.;以及应用柑桔黄龙病病原的特异性探针与其PCR 产物进行Southern 杂交试验,能产生杂交反应;这些都表明在柚树病原DNA与柑桔黄龙病病原DNA之间存在着十分高度的同源性.综上结果证明:柚类叶片斑驳的病原很可能与柑桔黄龙病病原是相同的,或其关系是十分密切的.
유류시감길중적일류중요품충,재아국유엄범적분포.근년래,재복건등산구발현유류병수일추증다,기병상위협편반박、소과、식주왜화,잠칭위협편반박병.본병능통과조직가접종병유수전염도유건묘화호감건묘.재전경하관찰,기병원균체적형태화세포벽구조여감길황룡병적병원(Liberobacter asiaticum)십분상사.응용감길황룡병병원DNA적특이성인물진행PCR시험산생적PCR 산물,기분자량여감길황룡병병원DNA PCR 산물적기본상동,위563 bp.;이급응용감길황룡병병원적특이성탐침여기PCR 산물진행Southern 잡교시험,능산생잡교반응;저사도표명재유수병원DNA여감길황룡병병원DNA지간존재착십분고도적동원성.종상결과증명:유류협편반박적병원흔가능여감길황룡병병원시상동적,혹기관계시십분밀절적.
Pomelo is a group of popular cultivars of citrus in China. In recent years, diseased plants of pomelo have been found in Fujian production areas. The symptoms of the diseased trees include leaf mottle, small fruit and stunting. The disease is graft-transmissible from diseased plant to healthy pomelo and ponkan via tissue grafting. The morphological characteristics of the pathogen resemble those of Liberobacter asiaticum which causes citrus Huanglongbing (formerly "citrus greening") based on electron microscopy. The PCR products of pathogen DNA obtained with gene-specific primers for Huanglongbing liberobacter and Southern hybridization of the PCR products with oligonucleotide probe specific for the liberobacter pathogen show that there is a remarkable degree of DNA homogeneity between the pathogen from diseased pomelo and that of citrus Huanglongbing plants. Taken together, the results of this study show that the causative agent of pomelo disease is either identical or very closely related to the Liberobacter asiaticum which causes citrus Huanglongbing.