CAJ | 학술논문

目的研究G蛋白β3亚单位825T等位基因与2型糖尿病及糖尿病肾病的相关性. 方法在单纯2型糖尿病(77例)和并发糖尿病肾病(71例)的患者中采用PCR-RFLP方法研究G蛋白β3亚单位C825T基因多态性,以正常人及原发性高血压患者为对照. 结果单纯糖尿病人群T等位基因(46.1%)及TT基因型频率(20.8%)稍高于对照组(T等位基因频率40.4%,TT基因型频率14.6%),但未达统计学显著意义,而糖尿病肾病组两种指标均明显高于对照组(P<0.05). 结论 G蛋白β3亚单位825T等位基因与糖尿病肾病相关,可能是糖尿病发病的候选基因.
목적연구G단백β3아단위825T등위기인여2형당뇨병급당뇨병신병적상관성. 방법재단순2형당뇨병(77례)화병발당뇨병신병(71례)적환자중채용PCR-RFLP방법연구G단백β3아단위C825T기인다태성,이정상인급원발성고혈압환자위대조. 결과단순당뇨병인군T등위기인(46.1%)급TT기인형빈솔(20.8%)초고우대조조(T등위기인빈솔40.4%,TT기인형빈솔14.6%),단미체통계학현저의의,이당뇨병신병조량충지표균명현고우대조조(P<0.05). 결론 G단백β3아단위825T등위기인여당뇨병신병상관,가능시당뇨병발병적후선기인.
Objective To investigate the relevance of the 825T allele of the G protein β3 sub-unit with diabetes mellitus and diabetic nephropathy in Chinese. Methods Participants comprised of 77 diabetes mellitus patients without any complications and 71 diabetes mellitus patients with nephropathy. 117 essential hypertensive patients and 151 normotensive control subjects without hypertension or diabetes mellitus family history acted as controls. DNA was extracted from whole blood. Genotype was measured by PCR-RFLP. Results Both T allele frequency and TT genotype were higher significantly in subjects with diabetic nephropathy than that in control group (P<0. 05). Conclusions The findings showed a possible association between the 825T allele and diabetic nephropathy which should be proved by more investigation and the Gβ3 gene may be a candidate gene of diabetes mellitus.