CAJ | 학술논문

　　为探究一氧化氮（NO）和水孔蛋白（AQP）在种子萌发过程中是否存在互作效应，以小麦品种陕合6号（耐旱型）和郑引1号（干旱敏感型）为材料，研究H2O、HgCl2（500μM）、PEG（PEG-8000，-0．35 MPa）、SNP（0．5 mM）、SNP＋PEG、SNP＋HgCl2溶液浸种对种子萌发的影响。结果表明：SNP（NO供体）促进了抗旱品种种子的萌发，而抑制了干旱敏感品种种子的萌发；AQP在干旱敏感品种种子萌发中的作用大于抗旱品种；NO能够解除干旱和水孔蛋白抑制剂HgCl2对种子萌发的抑制作用；处理对吸胀种子萌发的抑制效应小于直接在处理液中萌发的抑制效应；NO对AQP的活性具有调节作用且NO和AQP的作用主要存在于种子最初吸水萌动的12 h内。
　　위탐구일양화담（NO）화수공단백（AQP）재충자맹발과정중시부존재호작효응，이소맥품충협합6호（내한형）화정인1호（간한민감형）위재료，연구H2O、HgCl2（500μM）、PEG（PEG-8000，-0．35 MPa）、SNP（0．5 mM）、SNP＋PEG、SNP＋HgCl2용액침충대충자맹발적영향。결과표명：SNP（NO공체）촉진료항한품충충자적맹발，이억제료간한민감품충충자적맹발；AQP재간한민감품충충자맹발중적작용대우항한품충；NO능구해제간한화수공단백억제제HgCl2대충자맹발적억제작용；처리대흡창충자맹발적억제효응소우직접재처리액중맹발적억제효응；NO대AQP적활성구유조절작용차NO화AQP적작용주요존재우충자최초흡수맹동적12 h내。
In order to explore the interactions between nitric oxide (NO) and aquaporin (AQP) in seed germina-tion ,two wheat varieties including Shaanhe No .6 (SH6 ,drought-resistant) and Zhengying No .1 (ZY1 ,drought-suscep-tible) were adopted to study the effects of H2O ,HgCl2 (500μM) ,PEG (PEG-8000 ,-0 .35 MPa) ,SNP (0 .5 mM) , SNP+PEG and SNP+HgCl2 solution soaking on seed germination .The results showed that SNP (NO donor) promoted seed germination of the drought-resistant variety ,while inhibited that of the drought-susceptible variety .AQP played a more important role in the drought-susceptible variety than in the drought-tolerance variety .NO could relieve the inhibi-tion of seed germination caused by drought or aquaporin inhibitor (HgCl2 ) .The inhibition degree of stress to germination of the seeds being imbibed was smaller than that of the seeds being germinated directly .NO could regulate the activity of AQP ,and both NO and AQP exerted their role mainly in the initial 12 h of germination .