CAJ | 학술논문

建立了饲料中阿维菌素类药物(阿维菌素、多拉菌素、埃普菌素和伊维菌素)液相色谱-质谱(LC-MS)检测法. 用乙腈提取样品中的药物, 加水稀释, 加三乙胺调节pH, 经C18固相萃取柱净化, LC-MS法测定. 结果表明, 方法平均回收率为91.3%～99.8%, RSD为2.9%～15% (n=4), 配合饲料、浓缩饲料和预混合饲料中的检出限均为10 μg/kg; 定量限均为20 μg/kg. 方法可用于饲料样品中阿维菌素类药物残留量的确证检测.
건립료사료중아유균소류약물(아유균소、다랍균소、애보균소화이유균소)액상색보-질보(LC-MS)검측법. 용을정제취양품중적약물, 가수희석, 가삼을알조절pH, 경C18고상췌취주정화, LC-MS법측정. 결과표명, 방법평균회수솔위91.3%～99.8%, RSD위2.9%～15% (n=4), 배합사료、농축사료화예혼합사료중적검출한균위10 μg/kg; 정량한균위20 μg/kg. 방법가용우사료양품중아유균소류약물잔류량적학증검측.
An analytical method of avermectins (avermectin, dormectin, eprinomectin and ivermectin) was established by LC-MS. 4 kinds of avermectins were simply extracted with acetonitrile from feeds. Then, the extraction was diluted with water and pH was adjusted with triethylamine. Target compounds were selectively concentrated by C18 SPE cartridge. They were determined by LC-MS. The average recoveries for 4 avermectins were ranged from 91.3% to 99.8%. The coefficients of variation were in the range of 2.9%～15% (n=4). The limits of detections were 10 μg/kg and the quantitative limits were 20 μg/kg for complete feeds, concentrated feeds and premixed feeds. The method can be used for confirmation of the detection of avermectin residues in feeds.