安徽农业科学
安徽農業科學
안휘농업과학
JOURNAL OF ANHUI AGRICULTURAL SCIENCES
2009年
34期
16775-16777
,共3页
胰高血糖素样肽1受体%Exendin-4%易错PCR%噬菌体表面展示
胰高血糖素樣肽1受體%Exendin-4%易錯PCR%噬菌體錶麵展示
이고혈당소양태1수체%Exendin-4%역착PCR%서균체표면전시
Glucogan-like peptide 1 receptor%Exendin-4%Error-prone PCR%Phage display
[目的]通过噬菌体表面展示技术寻找胰高血糖素样肽1受体N端片段(nGLP-1R)结合Exendin-4的关键位点.[方法]通过易错PCR建立一个鼠肺nGLP-1R(从第21个氨基酸到第145个氨基酸)的噬菌体随机突变展示肽库.根据筛选出的突变体分析突变后nGLP-1R与Exendin-4结合活性.[结果] nGLP-1R第30位、第46位和第92位氨基酸是其与Exendin-4结合的潜在位点.[结论]关键位点单个氨基酸残基的突变可以改变nGLP-1R蛋白质的生物学活性.
[目的]通過噬菌體錶麵展示技術尋找胰高血糖素樣肽1受體N耑片段(nGLP-1R)結閤Exendin-4的關鍵位點.[方法]通過易錯PCR建立一箇鼠肺nGLP-1R(從第21箇氨基痠到第145箇氨基痠)的噬菌體隨機突變展示肽庫.根據篩選齣的突變體分析突變後nGLP-1R與Exendin-4結閤活性.[結果] nGLP-1R第30位、第46位和第92位氨基痠是其與Exendin-4結閤的潛在位點.[結論]關鍵位點單箇氨基痠殘基的突變可以改變nGLP-1R蛋白質的生物學活性.
[목적]통과서균체표면전시기술심조이고혈당소양태1수체N단편단(nGLP-1R)결합Exendin-4적관건위점.[방법]통과역착PCR건립일개서폐nGLP-1R(종제21개안기산도제145개안기산)적서균체수궤돌변전시태고.근거사선출적돌변체분석돌변후nGLP-1R여Exendin-4결합활성.[결과] nGLP-1R제30위、제46위화제92위안기산시기여Exendin-4결합적잠재위점.[결론]관건위점단개안기산잔기적돌변가이개변nGLP-1R단백질적생물학활성.
[Objective] To seek for the key binding sites of the N-terminal fragment of glucogan-like peptide 1 receptor (nGLP-1R) to exendin-4 through phage display.[Method] A randomly mutated phage display peptide library of the N-terminal (21-145 residues) extracellular domain of glucogan-like peptide 1 receptor from rat lung was constructed by error-prone PCR.With the obtained mutants,the binding activity of the nGLP-1R to exendin-4 was analyzed.[Result] The 30th,the 46th and the 92nd amino acids of the nGLP-1R were the potential sites binding to exendin-4.[Conclusion] The mutation of single amino acid at the key sites of nGLP-1R can change the biologic activity of the protein.
