化学试剂
化學試劑
화학시제
CHEMICAL REAGENTS
2009年
11期
913-914,947
,共3页
夏敏%安礼涛%尹起范%李茂青%邢华
夏敏%安禮濤%尹起範%李茂青%邢華
하민%안례도%윤기범%리무청%형화
高效液相色谱法%乙烯利%6-苄氨基嘌呤%测定
高效液相色譜法%乙烯利%6-芐氨基嘌呤%測定
고효액상색보법%을희리%6-변안기표령%측정
HPLC%ethephon%6-benzylaminopurine%determination
首次建立了高效液相色谱测定40%乙烯利中6-苄氨基嘌呤含量的方法.色谱柱为Symmetry C18(150 mm×3.9 mm×5 μm);流动相为甲醇-水(V/V=50:50);流速为0.8 mL/min;线性回归方程为y=9.35×10~6x-3.52×10~3,r=0.999 8,线性范围为32.9～411 μg;加标回收率平均值为98.2%,RSD为1.42%.
首次建立瞭高效液相色譜測定40%乙烯利中6-芐氨基嘌呤含量的方法.色譜柱為Symmetry C18(150 mm×3.9 mm×5 μm);流動相為甲醇-水(V/V=50:50);流速為0.8 mL/min;線性迴歸方程為y=9.35×10~6x-3.52×10~3,r=0.999 8,線性範圍為32.9～411 μg;加標迴收率平均值為98.2%,RSD為1.42%.
수차건립료고효액상색보측정40%을희리중6-변안기표령함량적방법.색보주위Symmetry C18(150 mm×3.9 mm×5 μm);류동상위갑순-수(V/V=50:50);류속위0.8 mL/min;선성회귀방정위y=9.35×10~6x-3.52×10~3,r=0.999 8,선성범위위32.9～411 μg;가표회수솔평균치위98.2%,RSD위1.42%.
A method for determining the content of 6-benzy-laminopurine in ethephon by HPLC was established for the first time. The column selected was waters symmetry C_(18)(150 mm × 3.9 mm×5 μm); methanol-water mixture (50 : 50, v/v) was used as the mobile phase;the flow rate was 0.8 mL/min and the detection wavelength was 270 nm.The linear equation was: y= 9.35× 0~6x-3.52×10~3(r = 0.999 8).The linear range for detection of 6-benzylaminopurine was 32.9μg～411μg ( r = 0.999 8). The average recovery of 6-benzylaminopurine was 98.2% with RSD= 1.42%.
