CAJ | 학술논문

目的比较香附经二氧化碳超临界流体萃取(SFE-CO_2)后药渣的5种方法提取液(SBE液、WE液、AE液、SBAE液、WAE液)对兔离体肠平滑肌的影响.方法以兔离体肠平滑肌收缩频率、幅度和张力为指标,采用BL-420E+生物机能实验系统,比较香附SFE-CO_2后药渣的5种方法提取液对正常及乙酰胆碱作用下的兔离体肠平滑肌的作用.结果香附SFE-CO_2后药渣的5种方法提取液均能显著降低正常兔离体肠运动的频率和张力 (P<0.05);降低乙酰胆碱作用下离体肠管的幅度及张力(P<0.01);其中SBE提取液与其他提取液相比,表现出更强的抑制作用(P<0.05).结论香附SFE-CO_2后药渣的5种方法提取液对兔离体肠平滑肌均具有抑制作用,其中SBE提取液的作用最强.
목적 비교향부경이양화탄초림계류체췌취(SFE-CO_2)후약사적5충방법제취액(SBE액、WE액、AE액、SBAE액 、WAE액)대토리체장평활기적영향.방법 이토리체장평활기수축빈솔、폭도화장력위지표,채용BL-420E+생물궤능실험계통,비교향부SFE-CO_2후약사적5충방법제취액대정상급을선담감작용하적토리체장평활기적작용.결과 향부SFE-CO_2후약사적5충방법제취액균능현저강저정상토리체장운동적빈솔화장력 (P<0.05);강저을선담감작용하리체장관적폭도급장력(P<0.01);기중SBE제취액여기타제취액상비,표현출경강적억제작용(P<0.05).결론 향부SFE-CO_2후약사적5충방법제취액대토리체장평활기균구유억제작용,기중SBE제취액적작용최강.
Objective To compare the post carbon dioxide supercritical fluid extraction (SFE-CO_2) effects of 5 kinds of drug extracts (SBE liquid,WE liquid,AE liquid,SBAE liquid,WAE liquid) Rhizoma Cyperi on the movement of in vitro intestine smooth muscle in drug.Methods The contractive amplitude, frequency, and tension of the normal and acetylcholine-induced rabbit intestine smooth muscle were recorded with BL-420E~+ biological and functional experimental system.Results Rhizoma Cyperi drug extracts could significantly reduce the frequency and tension of the normal rabbit intestine smooth muscle(P<0.05), depress the contractive amplitude and tension of intestine smooth muscle after acetylcholine induced hyperkinesias(P<0.01),in which the SBE extract group had the greatest effect (P<0.05). Conclusion All the Rhizoma Cyperi drug extracts can restrain the movement of rabbit intestine smooth muscle in vitro, and the effect of SBE group is the greatest than the other four groups.