CAJ | 학술논문

考察了4种大孔吸附树脂对仙人掌总黄酮的吸附分离性能,筛选出效果最佳的树脂为AB-8.以总黄酮的吸附量、总黄酮含量和回收率为考察指标,采用紫外分光光度法测定总黄酮.确定了AB-8树脂吸附分离仙人掌总黄酮的工艺条件:上样浓度为15mg/mL,仙人掌总黄酮最大吸附量为18.6mg/mL,吸附流速为5mL/min,洗脱剂为70%乙醇,洗脱剂用量为6倍柱体积,树脂可重复使用3次.经AB-8树脂分离纯化后,总黄酮含量从29%提高到76%,总黄酮回收率为86%.实验结果表明,AB-8树脂可用于仙人掌总黄酮的分离纯化.
고찰료4충대공흡부수지대선인장총황동적흡부분리성능,사선출효과최가적수지위AB-8.이총황동적흡부량、총황동함량화회수솔위고찰지표,채용자외분광광도법측정총황동.학정료AB-8수지흡부분리선인장총황동적공예조건:상양농도위15mg/mL,선인장총황동최대흡부량위18.6mg/mL,흡부류속위5mL/min,세탈제위70%을순,세탈제용량위6배주체적,수지가중복사용3차.경AB-8수지분리순화후,총황동함량종29%제고도76%,총황동회수솔위86%.실험결과표명,AB-8수지가용우선인장총황동적분리순화.
To assess the capability of 4 kinds of macroporus adsorption resins for absorbing and separating total flavone in cactus. The optimum adsorption results were got by AB-8 resin. The evaluation indexes included total flavone adsorption capacity, total flavone content, and recovery rate. The UV spectrophotometric method was used in determination of total flavone. The technological condition of adsorption and separating total flavone in opuntia dillenii Haw: the extract concentration was 15mg/mL, the maximum adsorption capacity was 18.6mg/mL, the adsorption velocity was 5mL/min, the eluting reagent was 70% ethanol, the dose of the reagent was 6 times as much as the column volume, and the resin could be used three times repeatedly. Total flavone content can be increased from 29% to 76%, with a total flawone recovery rate of 86% following the separation and purification by AB-8 resin. The results indicated that AB-8 resin can be used in separation and purification for total flavone in opuntia dillenii Haw.