中山大学学报（医学科学版）
中山大學學報（醫學科學版）
중산대학학보（의학과학판）
JOURNAL OF SUN YAT-SEN UNIVERSITY(MEDICAL SCIENCES)
2010年
2期
213-220
,共8页
邬继栋%何斯纯%黄雪松%沈伟哉
鄔繼棟%何斯純%黃雪鬆%瀋偉哉
오계동%하사순%황설송%침위재
黄皮核%N-甲基-N-顺式-苯乙烯基-桂皮酰胺%癫痫%青霉素诱发%痫样放电
黃皮覈%N-甲基-N-順式-苯乙烯基-桂皮酰胺%癲癇%青黴素誘髮%癇樣放電
황피핵%N-갑기-N-순식-분을희기-계피선알%전간%청매소유발%간양방전
Clausena lansium%N-methyl-N-cis-styryl-cinnamamide%Penicillin-induced%epilepsy%epileptiform discharges
[目的]探讨黄皮核提取物N-甲基-N--顺式-苯乙烯基-桂皮酰胺对青霉素(PNC)诱发大鼠癫痫的影响.[方法]32只SD大鼠随机分成正常对照组(20 mL/L吐温80+生理盐水)、模型对照组(20 mL/L吐温80+PNC)、低剂量桂皮酰胺组(桂皮酰胺75 mg/kg+PNC)、高剂量桂皮酰胺组(桂皮酰胺150 mg/kg+PNC),每组8只.建立大鼠皮层定位注射青霉素诱发惊厥模型,观察并分析两剂量的桂皮酰胺腹腔注射前处理给药对造模后大鼠惊厥发作程度和皮层脑电图的影响.[结果]两种剂量的,N-甲基-N-顺式-苯乙烯基-桂皮酰胺均可减轻大鼠癫痫发作程度,延长痫样放电的潜伏期,减少痫波持续时间,在发作后期减少痫波发放频率,与模型对照组比较,差异明显(P＜0.05或P＜0.01).然而,对于棘波最高和最低波幅的影响,桂皮酰胺组与模型对照组比较差异无统计学意义(P＞0.05).[结论]天然产物黄皮核提取物,N-甲基-N-顺式-苯乙烯基-桂皮酰胺对大鼠皮层定位注射青霉素诱发的惊厥发作和痫样放电具有明显的抑制作用,该天然产物具有一定的抗惊活性.
[目的]探討黃皮覈提取物N-甲基-N--順式-苯乙烯基-桂皮酰胺對青黴素(PNC)誘髮大鼠癲癇的影響.[方法]32隻SD大鼠隨機分成正常對照組(20 mL/L吐溫80+生理鹽水)、模型對照組(20 mL/L吐溫80+PNC)、低劑量桂皮酰胺組(桂皮酰胺75 mg/kg+PNC)、高劑量桂皮酰胺組(桂皮酰胺150 mg/kg+PNC),每組8隻.建立大鼠皮層定位註射青黴素誘髮驚厥模型,觀察併分析兩劑量的桂皮酰胺腹腔註射前處理給藥對造模後大鼠驚厥髮作程度和皮層腦電圖的影響.[結果]兩種劑量的,N-甲基-N-順式-苯乙烯基-桂皮酰胺均可減輕大鼠癲癇髮作程度,延長癇樣放電的潛伏期,減少癇波持續時間,在髮作後期減少癇波髮放頻率,與模型對照組比較,差異明顯(P＜0.05或P＜0.01).然而,對于棘波最高和最低波幅的影響,桂皮酰胺組與模型對照組比較差異無統計學意義(P＞0.05).[結論]天然產物黃皮覈提取物,N-甲基-N-順式-苯乙烯基-桂皮酰胺對大鼠皮層定位註射青黴素誘髮的驚厥髮作和癇樣放電具有明顯的抑製作用,該天然產物具有一定的抗驚活性.
[목적]탐토황피핵제취물N-갑기-N--순식-분을희기-계피선알대청매소(PNC)유발대서전간적영향.[방법]32지SD대서수궤분성정상대조조(20 mL/L토온80+생리염수)、모형대조조(20 mL/L토온80+PNC)、저제량계피선알조(계피선알75 mg/kg+PNC)、고제량계피선알조(계피선알150 mg/kg+PNC),매조8지.건립대서피층정위주사청매소유발량궐모형,관찰병분석량제량적계피선알복강주사전처리급약대조모후대서량궐발작정도화피층뇌전도적영향.[결과]량충제량적,N-갑기-N-순식-분을희기-계피선알균가감경대서전간발작정도,연장간양방전적잠복기,감소간파지속시간,재발작후기감소간파발방빈솔,여모형대조조비교,차이명현(P＜0.05혹P＜0.01).연이,대우극파최고화최저파폭적영향,계피선알조여모형대조조비교차이무통계학의의(P＞0.05).[결론]천연산물황피핵제취물,N-갑기-N-순식-분을희기-계피선알대대서피층정위주사청매소유발적량궐발작화간양방전구유명현적억제작용,해천연산물구유일정적항량활성.
[Objective]To investigate the effects of the seed extract of Clausena lansium,N-methyl-N-cis-styryl-cinnamamide,on the penicillin-induced epilepsy in rats.[Methods]Thirty-two Sprague-Dawely rata were randomly divided into four groups with 8 rats in each group:normal control(20 mL/L Tween 80+Normal Saline),model control(20 mL/L Tween 80+Penicillin),low dose treatment(Cinnamamide 75 mg/kg+Penicillin),hish dose treatment(Cinnamamide 150 mg/kg+Penicillin).The epilepsy rat model was established by localized intracortical injections of penicillin.The effects of pretreatments of the rats with N-methyl-N-cis-styryl-cinnamamide(intraperitoneal injection,ip)on the penicillin-induced seizure activities and the epileptiform discharges recorded by electrocorticogram(ECoG)were observed.[Results]The penicillin-induced seizure was significantly lightened in both the pretreatment groups(75 and 150 mg/kg N-methyl-N-cis-styryl-cinnamamide),compared with the model group.N-methyl-N-cis-styryl-cinnamamide(75 and 150 mg/kg)could significantly prolonged the latency of epileptiform activities,shorten the duration of epileptiform discharges and decreased the mean spike frequency in the late stage(P＜0.05 or P＜0.01).However,there was no significant difference in the highest value or the lowest value of the epileptiform waves between the model and cinnamamide treatment groups.[Conclusion]The seed extract of Clansena lansium,N-methyl-N-cis-styryl-cinnamamide,can significantly inhibit the seizure and epileptiform discharges caused by penicillin-localized injections in the cortex of rats,suggesting that the natural substance has an anticonvulsive function.
