农业科学与技术(英文版)
農業科學與技術(英文版)
농업과학여기술(영문판)
AGRICULTURAL SCIENCE & TECHNOLOGY
2010年
9期
82-84,90
,共4页
王敬东%张丽%马洪爱%陈晓军%宋玉霞
王敬東%張麗%馬洪愛%陳曉軍%宋玉霞
왕경동%장려%마홍애%진효군%송옥하
草莓花药%TDZ%愈伤组织%不定芽
草莓花藥%TDZ%愈傷組織%不定芽
초매화약%TDZ%유상조직%불정아
Strawberry anther%TDZ%Callus%Adventitious bud
[目的]探讨植物生长调节剂TDE在草莓花药培养中的应用.[方法]采用不同浓度TDZ与NAA植物生长素进行组合,观察其对草莓愈伤组织诱导及其分化的影响.[结果] MS+TDZ 1.0 mg/L+NAA 0.5 mg/L适用于草莓愈伤组织的分化,分化率高达75%,但TDZ对草莓愈伤组织的诱导无显著作用.[结论]该研究结果为草莓通过花药培养途径进行规模生产提供参考.
[目的]探討植物生長調節劑TDE在草莓花藥培養中的應用.[方法]採用不同濃度TDZ與NAA植物生長素進行組閤,觀察其對草莓愈傷組織誘導及其分化的影響.[結果] MS+TDZ 1.0 mg/L+NAA 0.5 mg/L適用于草莓愈傷組織的分化,分化率高達75%,但TDZ對草莓愈傷組織的誘導無顯著作用.[結論]該研究結果為草莓通過花藥培養途徑進行規模生產提供參攷.
[목적]탐토식물생장조절제TDE재초매화약배양중적응용.[방법]채용불동농도TDZ여NAA식물생장소진행조합,관찰기대초매유상조직유도급기분화적영향.[결과] MS+TDZ 1.0 mg/L+NAA 0.5 mg/L괄용우초매유상조직적분화,분화솔고체75%,단TDZ대초매유상조직적유도무현저작용.[결론]해연구결과위초매통과화약배양도경진행규모생산제공삼고.
[Objective] The aim was to investigate the application of plant growth regulator thidiazuron (TDZ) in strawberry anther culture. [Method] Different combinations of TDZ and naphthalene acetic acid (NAA) auxine with different concentrations were used for induction culture, and then its effect on callus induction and plant regeneration were observed. [Result] The combination of 1.0 mg/L TDZ+0.5 mg/LNAA was effective to accelerating the differentiation and regeneration of strawberries, and the differentiation ratio was as high as 75%. However, TDZ showed insignificant effect on strawberries anther induction. [Conclusion] This research had provided reference for large-scale production of strawberry through anther culture.