中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2012年
5期
388-391
,共4页
左绪宏%史学锋%谢芳%许丽敏%赵堪兴
左緒宏%史學鋒%謝芳%許麗敏%趙堪興
좌서굉%사학봉%사방%허려민%조감흥
外侧膝状体%时空频率调谐%时间频率%空间频率%感受野
外側膝狀體%時空頻率調諧%時間頻率%空間頻率%感受野
외측슬상체%시공빈솔조해%시간빈솔%공간빈솔%감수야
Lateral geniculate neuron%Spatiotemporal frequency tuning%Temporal frequency%Spatial frequency%Receptive field
背景 外侧膝状体(LGN)是视觉通路中重要的视觉信息处理器官,近年来发现LGN参与运动视觉信息处理,功能磁共振成像检查表明在LGN水平存在着双眼竞争,可能与弱视的发生有关.然而,LGN神经元时空频率特性的研究多限于猴或者猫,大鼠的相关研究较少. 目的 观察不同年龄段大鼠LGN神经元感受野时空频率调谐特性.方法 取睁眼后14～16d组、20 ～ 22 d组、27 ～30d组及睁眼后60 d组Wistar 大鼠各5只,用在体细胞外记录技术,通过正弦光栅视觉刺激分别对各组大鼠LGN神经元感受野时空调谐特性进行检测,观察大鼠LGN神经元时空频率调谐特性随着发育发生的动态变化. 结果 睁眼后14～16 d组、20～22 d组、27 ～30 d组及睁眼后60d组大鼠LGN神经元时间和空间频率调谐低通调制和带通调制间神经元数量的差异均无统计学意义(x2=0.68、0.47,P＞0.05),4个组大鼠LGN神经元感受野最优时间频率调谐特性成年后达最高值,大鼠LGN神经元的最优时间频率均值分别为(2.5±1.3)、(2.6±1.2)、(2.6±1.1)、(3.6±1.1)Hz,差异有统计学意义(F=4.53,P＜0.05),睁眼后60d组的最优时间频率明显高于睁眼后14～16 d组、20 ～22 d组、27 ～30 d组,差异均有统计学意义(q=4.43、4.10、4.03,P＜0.05),睁眼后14 ～ 16 d组、20～22 d组、27 ～30 d组间的相互比较差异均无统计学意义(P＞0.05).4个组大鼠LGN神经元的最优空间频率均值分别为(0.04±0.04)、(0.04±0.03)、(0.05±0.03)、(0.05士0.04)cpd,差异均无统计学意义(F=0.58,P＞0.05),而时间频率瞬时带宽及空间频率带宽各年龄组间比较,差异均无统计学意义(F=0.37、1.22,P＞0.05).结论大鼠LGN神经元感受野时空频率调谐发育特性的不同可能与其在视觉通路中的功能有关.
揹景 外側膝狀體(LGN)是視覺通路中重要的視覺信息處理器官,近年來髮現LGN參與運動視覺信息處理,功能磁共振成像檢查錶明在LGN水平存在著雙眼競爭,可能與弱視的髮生有關.然而,LGN神經元時空頻率特性的研究多限于猴或者貓,大鼠的相關研究較少. 目的 觀察不同年齡段大鼠LGN神經元感受野時空頻率調諧特性.方法 取睜眼後14～16d組、20 ～ 22 d組、27 ～30d組及睜眼後60 d組Wistar 大鼠各5隻,用在體細胞外記錄技術,通過正絃光柵視覺刺激分彆對各組大鼠LGN神經元感受野時空調諧特性進行檢測,觀察大鼠LGN神經元時空頻率調諧特性隨著髮育髮生的動態變化. 結果 睜眼後14～16 d組、20～22 d組、27 ～30 d組及睜眼後60d組大鼠LGN神經元時間和空間頻率調諧低通調製和帶通調製間神經元數量的差異均無統計學意義(x2=0.68、0.47,P＞0.05),4箇組大鼠LGN神經元感受野最優時間頻率調諧特性成年後達最高值,大鼠LGN神經元的最優時間頻率均值分彆為(2.5±1.3)、(2.6±1.2)、(2.6±1.1)、(3.6±1.1)Hz,差異有統計學意義(F=4.53,P＜0.05),睜眼後60d組的最優時間頻率明顯高于睜眼後14～16 d組、20 ～22 d組、27 ～30 d組,差異均有統計學意義(q=4.43、4.10、4.03,P＜0.05),睜眼後14 ～ 16 d組、20～22 d組、27 ～30 d組間的相互比較差異均無統計學意義(P＞0.05).4箇組大鼠LGN神經元的最優空間頻率均值分彆為(0.04±0.04)、(0.04±0.03)、(0.05±0.03)、(0.05士0.04)cpd,差異均無統計學意義(F=0.58,P＞0.05),而時間頻率瞬時帶寬及空間頻率帶寬各年齡組間比較,差異均無統計學意義(F=0.37、1.22,P＞0.05).結論大鼠LGN神經元感受野時空頻率調諧髮育特性的不同可能與其在視覺通路中的功能有關.
배경 외측슬상체(LGN)시시각통로중중요적시각신식처리기관,근년래발현LGN삼여운동시각신식처리,공능자공진성상검사표명재LGN수평존재착쌍안경쟁,가능여약시적발생유관.연이,LGN신경원시공빈솔특성적연구다한우후혹자묘,대서적상관연구교소. 목적 관찰불동년령단대서LGN신경원감수야시공빈솔조해특성.방법 취정안후14～16d조、20 ～ 22 d조、27 ～30d조급정안후60 d조Wistar 대서각5지,용재체세포외기록기술,통과정현광책시각자격분별대각조대서LGN신경원감수야시공조해특성진행검측,관찰대서LGN신경원시공빈솔조해특성수착발육발생적동태변화. 결과 정안후14～16 d조、20～22 d조、27 ～30 d조급정안후60d조대서LGN신경원시간화공간빈솔조해저통조제화대통조제간신경원수량적차이균무통계학의의(x2=0.68、0.47,P＞0.05),4개조대서LGN신경원감수야최우시간빈솔조해특성성년후체최고치,대서LGN신경원적최우시간빈솔균치분별위(2.5±1.3)、(2.6±1.2)、(2.6±1.1)、(3.6±1.1)Hz,차이유통계학의의(F=4.53,P＜0.05),정안후60d조적최우시간빈솔명현고우정안후14～16 d조、20 ～22 d조、27 ～30 d조,차이균유통계학의의(q=4.43、4.10、4.03,P＜0.05),정안후14 ～ 16 d조、20～22 d조、27 ～30 d조간적상호비교차이균무통계학의의(P＞0.05).4개조대서LGN신경원적최우공간빈솔균치분별위(0.04±0.04)、(0.04±0.03)、(0.05±0.03)、(0.05사0.04)cpd,차이균무통계학의의(F=0.58,P＞0.05),이시간빈솔순시대관급공간빈솔대관각년령조간비교,차이균무통계학의의(F=0.37、1.22,P＞0.05).결론대서LGN신경원감수야시공빈솔조해발육특성적불동가능여기재시각통로중적공능유관.
Background Recent researches suggested that properties of neurons in the lateral geniculate neuron (LGN) may represent an important neural limitation on the development of basic spatial and temporal vision,and even binocular rivalry.However,previous studies on the properties of spatiotemporal frequency tuning of LGN were rather concentrated on a monkey or cat,whereas little is known about rat. Objective This study was to examine the development of spatiotemporal frequency tuning in rats LGN. Methods Twenty Wistar rats were collected and divided into 14-16 day,20-22 day,27-30 day and 60 day groups according to the different ages after their eyes opened and 5 rats were assigned for each group.Extracellular single neuron recording was carried out in the rats to study the spatio-temporal receptive field properties of neurons in LGN by sinusoidal gratings visual stimuli.Dynamic changes of the spatio-temporal receptive field properties of neurons in LGN with the development of Wistar rats were evaluated. Results The differences between band-pass and low-pass distribution of temporal frequency or spatial frequency of rat LGN were not statistically significant (x2 =0.68,0.47,P＞0.05 ).The optimal temporal frequency of receptive field in rat LGN went up to the maximum value until 60 day in Wistar rats.The mean optimal temporal frequencies of neurons in the four different age groups were ( 2.5 ± 1.3 ),( 2.6± 1.2),(2.6± 1.1 ) and ( 3.6± 1.1 ) Hz with significant differences among the 4 groups (F=4.53,P＜0.05 ),and those in the 14-16 day group,20-22 day group,27- 30 day group were significantly lower than in the 60 days group ( q =4.43,4.10,4.03,P ＜ 0.05 ).No significant differences were seen among the 14-16 day group,20-22 day group and 27-30 day group ( P＞0.05 ).The optimal spatial frequency values in the four groups were ( 0.04 ± 0.04 ),( 0.04 ± 0.03 ),( 0.05 ± 0.03 ),( 0.05 ± 0.04 ) cpd,respectively without statistical difference among them ( F =0.58,P ＞ 0.05 ).The temporal and spatial bandwidth values in the various age groups were not statistically significant among the four groups ( F =0.37,1.22,P＞0.05). Conclusions The development of temporal and spatial frequency characteristics of the rat LGN receptive field may be related to its functional visual pathway.
