中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2012年
1期
37-39
,共3页
刘芳芳%沈丹华%王杉%叶颖江%丘辉%王成刚%张彦斌%宋秋静
劉芳芳%瀋丹華%王杉%葉穎江%丘輝%王成剛%張彥斌%宋鞦靜
류방방%침단화%왕삼%협영강%구휘%왕성강%장언빈%송추정
结直肠肿瘤%基因%基因表达%肿瘤标记,生物学%免疫疗法
結直腸腫瘤%基因%基因錶達%腫瘤標記,生物學%免疫療法
결직장종류%기인%기인표체%종류표기,생물학%면역요법
Colorectal neoplasms%Genes%Gene expression%Tumor markers,biological%Immunotherapy
目的 探讨MAGE-A3、MAGE-A4、MAGE-A10基因在结直肠原发腺癌组织中的蛋白表达及临床意义.方法 采用组织芯片技术、免疫组化方法分析多种MAGE基因在97例结直肠原发腺癌配对癌组织、癌旁组织中的蛋白表达.统计学方法采用x2检验.结果 MAGE-A3,MAGE-A4,MAGE-A10蛋白在97例结直肠原发腺癌组织中的表达率分别为57％(56/97),63％(61/97),28％(27/97).MAGE-A3在低分化腺癌中的表达率明显高于中分化和高分化腺癌中的表达率(x2=9.133,P=0.010).MAGE-A10在低分化腺癌中的表达率明显高于中分化和高分化腺癌(x2=15.280,P=0.000)；在TNMⅢ+Ⅳ期中的表达率明显高于Ⅰ+Ⅱ期(x2=4.227,P=0.040)；在有淋巴结转移原发灶中的表达率明显高于无淋巴结转移原发灶(x2 =5.557,P=0.018)；且随淋巴结转移数目的增多在原发灶表达率显著增高(x2=7.296,P=0.026).结论 MAGE基因的蛋白表达与结直肠癌的病理组织学分化、TNM分期、癌淋巴结转移有关.MAGE-A3和MAGE-A10可能成为结直肠癌的预后标志物及结直肠癌免疫治疗的候选靶点.
目的 探討MAGE-A3、MAGE-A4、MAGE-A10基因在結直腸原髮腺癌組織中的蛋白錶達及臨床意義.方法 採用組織芯片技術、免疫組化方法分析多種MAGE基因在97例結直腸原髮腺癌配對癌組織、癌徬組織中的蛋白錶達.統計學方法採用x2檢驗.結果 MAGE-A3,MAGE-A4,MAGE-A10蛋白在97例結直腸原髮腺癌組織中的錶達率分彆為57％(56/97),63％(61/97),28％(27/97).MAGE-A3在低分化腺癌中的錶達率明顯高于中分化和高分化腺癌中的錶達率(x2=9.133,P=0.010).MAGE-A10在低分化腺癌中的錶達率明顯高于中分化和高分化腺癌(x2=15.280,P=0.000)；在TNMⅢ+Ⅳ期中的錶達率明顯高于Ⅰ+Ⅱ期(x2=4.227,P=0.040)；在有淋巴結轉移原髮竈中的錶達率明顯高于無淋巴結轉移原髮竈(x2 =5.557,P=0.018)；且隨淋巴結轉移數目的增多在原髮竈錶達率顯著增高(x2=7.296,P=0.026).結論 MAGE基因的蛋白錶達與結直腸癌的病理組織學分化、TNM分期、癌淋巴結轉移有關.MAGE-A3和MAGE-A10可能成為結直腸癌的預後標誌物及結直腸癌免疫治療的候選靶點.
목적 탐토MAGE-A3、MAGE-A4、MAGE-A10기인재결직장원발선암조직중적단백표체급림상의의.방법 채용조직심편기술、면역조화방법분석다충MAGE기인재97례결직장원발선암배대암조직、암방조직중적단백표체.통계학방법채용x2검험.결과 MAGE-A3,MAGE-A4,MAGE-A10단백재97례결직장원발선암조직중적표체솔분별위57％(56/97),63％(61/97),28％(27/97).MAGE-A3재저분화선암중적표체솔명현고우중분화화고분화선암중적표체솔(x2=9.133,P=0.010).MAGE-A10재저분화선암중적표체솔명현고우중분화화고분화선암(x2=15.280,P=0.000)；재TNMⅢ+Ⅳ기중적표체솔명현고우Ⅰ+Ⅱ기(x2=4.227,P=0.040)；재유림파결전이원발조중적표체솔명현고우무림파결전이원발조(x2 =5.557,P=0.018)；차수림파결전이수목적증다재원발조표체솔현저증고(x2=7.296,P=0.026).결론 MAGE기인적단백표체여결직장암적병리조직학분화、TNM분기、암림파결전이유관.MAGE-A3화MAGE-A10가능성위결직장암적예후표지물급결직장암면역치료적후선파점.
Objective To explore protein expression and significance of MAGE genes in colorectal carcinoma(CRC)tissues.Methods The expression of MAGE genes were studied by using tissue chip and immunochemistry methods in primary CRC tissue and paired adjacent tissue samples in 97 cases.Data were analyzed with x2-test by SPSS 16.0 software.Results The protein expression of MAGE-A3,MAGE-A4 and MAGE-A10 genes were 57％(56/97),63％(61/97)and 28％(27/97)respectively in 97 cases of primary adenocarcinoma.The protein expression frequency of MAGE-A3 in poor colorectal adenocarcinomas was significantly higher than in well-and moderately disfferentiated adenocarcinomas(x2 =9.133,P =0.010).MAGE-A10 in poor colorectal primary adenocarcinomas was significantly higher than in well and moderately adenocarcinomas(x2 =15.280,P =0.000); MAGE-A10 protein expression was significantly higher in stage TNM Ⅲ + Ⅳ than in stage TNM Ⅰ + Ⅱ(x2 =4.227,P=0.040); MAGE-A10 gene expression was higher in metastasis lymphoid node than in no metastasis lymphoid node(x2 =5.557,P =0.018),and the expression level was higher in primary lesion with the increasing of the numbers of lymphoid node metastasis(x2 =7.296,P =0.026).Conclusions The protein expression of MAGE genes is associated with the tumor differentiation,TNM stage and lymphoid node metastasis.MAGE-A3 and MAGE-A10 genes are the possible prognosis marker and potential target of immunotherapy of CRC.
