CAJ | 학술논문

目的探讨大鼠出生后不同时期使用氟西汀对其体质量和行为的远期影响.方法随机选择雄性SPrague-Dawley大鼠,在其出生后1～7 d、8～21 d分别皮内注射氟西汀(浓度2 g/L,注射剂量5 ml/kg体质量)(F1组,22只;F2组,20只)和生理盐水(0.9%NaCl,注射剂量5 ml/kg体质量)(S1组,20只;S2组,19只),并追踪观察4组大鼠体质量;大鼠成年后(出生后第90天)进行行为学检测,包括旷场实验、高架十字迷宫、新奇抑制摄食和强迫游泳实验.结果 (1)F1组大鼠体质量的增加延缓,出生后第25天,F1组体质量[(35.5±3.4)g]于S1组[(43.0±3.9)g],至出生后第90天,F1组体质量[(190.7±12.1)g]均小于S1组[(208.0±13.5)g]和F2组[(218.3±14.6)g](两样本t检验,P＜0.05).(2)幼鼠早期使用氟西汀,成年后探索性行为减少,F1组旷场行为总行程[(18.9±2.3)m]明显小于S1组[(38.9±8.1)m],F2组[(33.3±6.2)m]于S2组[(43.7±6.2)m];高架十字迷宫总穿臂次数F1组[(13.8±3.2)次]少于S1组[(37.6±6.3)次],F2组[(32.3±7.1)次]少于S2组[(57±7.3)次](两样本t检验,P＜0.05);焦虑抑郁相关行为增加,新奇抑制摄食潜伏期F1组[(432.2±45.4)s]长于S1组[(167.7±20.3)s],F2组[(270.2±27.2)s]长于S2组[(185.3±19.2)s];强迫游泳静止不动时间百分比F1组[(41.2±3.2)%]长于S1组[(26.5±2.3)%],F2组[(35.1±3.6)%]长于S2组[(27.8±2.5)%](两样本t检验,P＜0.05);且F1组大鼠的异常行为重于F2组(两样本t检验,P＜0.05).结论幼鼠出生后早期使用氟西汀可导致大鼠体质量增加延缓,成年后出现焦虑抑郁行为,使用氟西汀越早风险越大.
목적 탐토대서출생후불동시기사용불서정대기체질량화행위적원기영향.방법 수궤선택웅성SPrague-Dawley대서,재기출생후1～7 d、8～21 d분별피내주사불서정(농도2 g/L,주사제량5 ml/kg체질량)(F1조,22지;F2조,20지)화생리염수(0.9%NaCl,주사제량5 ml/kg체질량)(S1조,20지;S2조,19지),병추종관찰4조대서체질량;대서성년후(출생후제90천)진행행위학검측,포괄광장실험、고가십자미궁、신기억제섭식화강박유영실험.결과 (1)F1조대서체질량적증가연완,출생후제25천,F1조체질량[(35.5±3.4)g]우S1조[(43.0±3.9)g],지출생후제90천,F1조체질량[(190.7±12.1)g]균소우S1조[(208.0±13.5)g]화F2조[(218.3±14.6)g](량양본t검험,P＜0.05).(2)유서조기사용불서정,성년후탐색성행위감소,F1조광장행위총행정[(18.9±2.3)m]명현소우S1조[(38.9±8.1)m],F2조[(33.3±6.2)m]우S2조[(43.7±6.2)m];고가십자미궁총천비차수F1조[(13.8±3.2)차]소우S1조[(37.6±6.3)차],F2조[(32.3±7.1)차]소우S2조[(57±7.3)차](량양본t검험,P＜0.05);초필억욱상관행위증가,신기억제섭식잠복기F1조[(432.2±45.4)s]장우S1조[(167.7±20.3)s],F2조[(270.2±27.2)s]장우S2조[(185.3±19.2)s];강박유영정지불동시간백분비F1조[(41.2±3.2)%]장우S1조[(26.5±2.3)%],F2조[(35.1±3.6)%]장우S2조[(27.8±2.5)%](량양본t검험,P＜0.05);차F1조대서적이상행위중우F2조(량양본t검험,P＜0.05).결론 유서출생후조기사용불서정가도치대서체질량증가연완,성년후출현초필억욱행위,사용불서정월조풍험월대.
Objective To evaluate the long-term influence of fluoxetine exposure at different neonate period on the body weight and behavior in rats. Methods Male SPrague-Dawley rats were randomized to be treated with fluoxetine (concentration: 2 g/L, 5 ml/kg) (F1 :n =22,F2:n =20)or saline (0. 9% NaCl, 5 ml/kg) (S1 :n =20,S2:n = 19) on days 1 -7 or days 8 -21 after birth. The body weights of rats in all 4 groups were trailed. Ethological tests, including open field test, elevated-plus maze, noveltysuppressed feeding test and forced swim test, were performed in the adult rats (90 days after birth). Results ( 1 ) Weight gain was delayed to 25 days after birth in F1 group, and was lower [ ( 35.5 ± 3.4 ) g ] in comparison to group S1 [ (43. 0 ± 3.9) g], and was [ (190. 7 ± 12. 1 ) g] lower than that in group S1[ (208.0±13.5) g] and group F2 [218.3 ± 14.6) g] on days 90 (P ＜0.05). (2) Early fluoxetine exposure in neonate led to reduced exploratory behavior in adult rats. The total distance traveled in open field was significantly shorter in group F1 [ ( 18.9 ± 2. 3) m] than in group S1 [ (38. 9 ± 8. 1 ) m], and shorter in group F2 [ (33. 3 ± 6. 2) m ] than in group S2 [ (43.7 ± 6. 2 ) m ]. The total entries in elevated plus-maze was fewer in group F1 ( 13. 8 ± 3.2) than in group S1 ( 37. 6 ± 6. 3 ), and fewer in group F2 ( 32. 3 ± 7. 1 )than in group S2 (57 ± 7.3 ) ( P ＜ 0. 05 ). Depression anxiety behavior increased in adult rats with neonatal fluoxetine exposure, the latency in novelty-suppressed feeding test was longer in group F1 [ (432. 2 ±45.4)s] than in group S1[ (167.7 ±20.3) s], and longer in group F2[ (270. 2 ±27.2) s] than in group S2[ ( 185.3 ± 19. 2) s], the percentage observations of immobility in forced swim test was higher in group F1[ (41.2 ± 3. 2 )% ] than in group S1 [ (26. 5 ± 2. 3 )% ], and in group F2 [ (35. 1 ± 3.6 )% ] than in group S2 [ ( 27. 8± 2. 5 ) % ] ( P ＜ 0. 05 ) . Abnormal behavior was severer in group F1 than in group F2(P ＜0. 05). Conclusions Early exposure to fluoxetine after birth may result in slower weight gain and depression anxiety behavior in adult rats, the earlier fluoxetine exposure, the higher risk.