中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2010年
3期
152-154
,共3页
癌,鳞状细胞%微小RNA%细胞生长过程
癌,鱗狀細胞%微小RNA%細胞生長過程
암,린상세포%미소RNA%세포생장과정
Carcinoma,squamous cell%MicroRNA%Cell growth processes
目的 探讨微小RNA(microRNA,miRNA)对体外培养的人舌鳞状细胞癌TeaS113细胞增殖的影响.方法 用脂质体把miRNA-31和miRNA-139的类似物或抑制物分别导人人舌鳞状细胞癌Tca8113细胞,分别为实验组,脂质体试剂为对照组,然后用甲基噻唑基四唑(MTT)法检测细胞增殖情况.结果 对照组吸光度值(A值)在24、48和72 h时分别为(0.125±0.002)、(0.169±0.002)和(0.216 4±0.004);miRNA-31类似物可促进Tca8113细胞增殖,使其A值分别增至(0.136±0.001)(P<0.001)、(0.186±0.004)(P<0.001)和(0.249±0.012)(P<0.01);miRNA-139抑制物也使A值分别增加到(0.148±0.002)(P<0.001)、(0.214±0.002)(P<0.001)和(0.250±0.009)(P<0.01).与此相反,miRNA-31抑制物在48、72 h时抑制Tea8113细胞增殖,其A值分别降至(0.145±0.001)和(0.155±0.011)(P<0.001);mjRNA-139类似物在24、48 h也分别使A值降至(0.135±0.001)和(0.170±0.009)(P<0.001).结论 miRNA-31和miRNA-139在人舌鳞状细胞癌发生过程中起着重要作用,调整其活性有可能成为一种有效治疗口腔癌的新方法.
目的 探討微小RNA(microRNA,miRNA)對體外培養的人舌鱗狀細胞癌TeaS113細胞增殖的影響.方法 用脂質體把miRNA-31和miRNA-139的類似物或抑製物分彆導人人舌鱗狀細胞癌Tca8113細胞,分彆為實驗組,脂質體試劑為對照組,然後用甲基噻唑基四唑(MTT)法檢測細胞增殖情況.結果 對照組吸光度值(A值)在24、48和72 h時分彆為(0.125±0.002)、(0.169±0.002)和(0.216 4±0.004);miRNA-31類似物可促進Tca8113細胞增殖,使其A值分彆增至(0.136±0.001)(P<0.001)、(0.186±0.004)(P<0.001)和(0.249±0.012)(P<0.01);miRNA-139抑製物也使A值分彆增加到(0.148±0.002)(P<0.001)、(0.214±0.002)(P<0.001)和(0.250±0.009)(P<0.01).與此相反,miRNA-31抑製物在48、72 h時抑製Tea8113細胞增殖,其A值分彆降至(0.145±0.001)和(0.155±0.011)(P<0.001);mjRNA-139類似物在24、48 h也分彆使A值降至(0.135±0.001)和(0.170±0.009)(P<0.001).結論 miRNA-31和miRNA-139在人舌鱗狀細胞癌髮生過程中起著重要作用,調整其活性有可能成為一種有效治療口腔癌的新方法.
목적 탐토미소RNA(microRNA,miRNA)대체외배양적인설린상세포암TeaS113세포증식적영향.방법 용지질체파miRNA-31화miRNA-139적유사물혹억제물분별도인인설린상세포암Tca8113세포,분별위실험조,지질체시제위대조조,연후용갑기새서기사서(MTT)법검측세포증식정황.결과 대조조흡광도치(A치)재24、48화72 h시분별위(0.125±0.002)、(0.169±0.002)화(0.216 4±0.004);miRNA-31유사물가촉진Tca8113세포증식,사기A치분별증지(0.136±0.001)(P<0.001)、(0.186±0.004)(P<0.001)화(0.249±0.012)(P<0.01);miRNA-139억제물야사A치분별증가도(0.148±0.002)(P<0.001)、(0.214±0.002)(P<0.001)화(0.250±0.009)(P<0.01).여차상반,miRNA-31억제물재48、72 h시억제Tea8113세포증식,기A치분별강지(0.145±0.001)화(0.155±0.011)(P<0.001);mjRNA-139유사물재24、48 h야분별사A치강지(0.135±0.001)화(0.170±0.009)(P<0.001).결론 miRNA-31화miRNA-139재인설린상세포암발생과정중기착중요작용,조정기활성유가능성위일충유효치료구강암적신방법.
Objective To investigate the effects of microRNA(miRNA)on proliferation of cultured human squamous cell carcinoma of tongue Tca8113 cells.Methods The mimics or inhibitors of miRNA-31 or miRNA-139 were transfected into Tca8113 cells using liposome.Tca8113 cell proliferation was detected by 3-(4,5-dimethyhhiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.Results The absorbance (A) values of control group at 24 h,48 h and 72 h were 0.125±0.002,0.169±0.002 and 0.216±0.004.respectively.The mimics of miRNA-31 increased Tca8113 cell proliferation.with A values inereasing to 0.136±0.001(P<0.001).0.186±0.004(P<0.001)and 0.249±0.012(P<0.01),respectively.The inhibitors of miRNA-139 also inereased A values to 0.148±0.002(P<0.001).0.214±0.002(P<0.001)and 0.250±0.009(P<0.01),respectively.Contrast with these results,the inhibitors of miRNA-31 decreased Tca8113 cell proliferation,with A values decreasing to 0.145 4-0.001 and 0.155±0.011 (both of P<0.001) at 48 h and 72 h,respectively.The mimics of miRNA-139 also decreased A to 0.135±0.001 and 0.170±0.009 (both of P<0.001).Conclusions miRNA-31 and miRNA-139 play an important role in the carcinogenesis of human tongue carcinomas.It may become a new method for the treatment of tongue carcinomas by adjustment the activities of miRNA.
