CAJ | 학술논문

目的探讨BDNF - TrkB信号传导通路与杏仁核电刺激点燃大鼠Arc表达及癫痫发生的关系.方法雄性SD大鼠随机分为空白组、假手术组、杏仁核点燃组(单纯点燃组、k252a注射组、DMSO对照组).应用Alpha - Lab 4通道信号采集及处理系统记录大鼠脑电活动.运用免疫组化检测其Arc蛋白的表达水平,采用RT - PCR、原位杂交检测Arc mRNA在海马中表达水平.结果 k252a注射组大鼠V级发作持续时间(33.00±1.41)8显著低于单纯点燃组(60.50±2.07)s、DMSO对照组(60.00±1.79)s大鼠V级发作持续时间(P＜0.01).k252a注射组3h、6hArc阳性细胞百分率显著低于单纯点燃组、DMSO对照组3h、6hArc阳性细胞百分率(P＜0.01),12 h时5组间Arc阳性细胞百分率相比表达差异无统计学意义(F =0.684,P＞0.05).RT - PCR、原位杂交结果显示:k252a注射组1h、3 h Arc mRNA表达量显著低于单纯点燃组、DMSO对照组1h、3 h Arc mRNA表达量(P＜0.01).6h时5组间Arc mRNA相比表达差异无统计学意义(P＞0.05).结论 k252a能够抑制BDNF - TrkB 信号传导通路对Arc表达的诱导作用从而使癫痫发作的持续时间缩短.
목적 탐토BDNF - TrkB신호전도통로여행인핵전자격점연대서Arc표체급전간발생적관계.방법 웅성SD대서수궤분위공백조、가수술조、행인핵점연조(단순점연조、k252a주사조、DMSO대조조).응용Alpha - Lab 4통도신호채집급처리계통기록대서뇌전활동.운용면역조화검측기Arc단백적표체수평,채용RT - PCR、원위잡교검측Arc mRNA재해마중표체수평.결과 k252a주사조대서V급발작지속시간(33.00±1.41)8현저저우단순점연조(60.50±2.07)s、DMSO대조조(60.00±1.79)s대서V급발작지속시간(P＜0.01).k252a주사조3h、6hArc양성세포백분솔현저저우단순점연조、DMSO대조조3h、6hArc양성세포백분솔(P＜0.01),12 h시5조간Arc양성세포백분솔상비표체차이무통계학의의(F =0.684,P＞0.05).RT - PCR、원위잡교결과현시:k252a주사조1h、3 h Arc mRNA표체량현저저우단순점연조、DMSO대조조1h、3 h Arc mRNA표체량(P＜0.01).6h시5조간Arc mRNA상비표체차이무통계학의의(P＞0.05).결론 k252a능구억제BDNF - TrkB 신호전도통로대Arc표체적유도작용종이사전간발작적지속시간축단.
Objective To study the relationship of BDNF - TrkB signaling pathway and the expression of Arc in amygdala electrical stimulation kindled rats and the epilepsy.Methods Male SD rats were divided randomly into control group,sham group and amygdala kindled group (simple kindling group,k252a injection group,DMSO control group).Application of Alpha - Lab 4 channel signal acquisition and processing system recording the EEG activity of rats.The expression level of Arc gene in hippocampus was test by RT - PCR and hybridization,The expression level of Arc protein was test by immunohistochemistry.Results The V class insultus duration of k252a injection group rats (33.00 ± 1.41 )s,they were significantly less than the simple kindling group rats(60.50 ± 2.07) s and the DMSO control group rats(60.00 ± 1.79) s( P ＜0.01 ).The Arc of positive cells percentage was significantly lower at 3 h,6 h in k252a injection group than simple kinding group,DMSO control group( P ＜ 0.01 ).The expression of Arc positive cells percentage among 5 groups at 12 h was no statistically significant ( F =0.684,P ＞ 0.05 ).The resulted of RT - PCR,hybridization showing:The expression of Arc mRNA at 1 h,3 hsignificantly lower in k252a injection group than simple kinding group,DMSO control group( P ＜ 0.01 )The defference of expression of Arc mRNA at 6 h among 5 groups was no statistically significant ( P ＞0.05 ).Conclusion k252a can inhibiting BDNF- TrkB signaling pathyway inducing the expression of Arc,to reduce the duration of seizure.